Kolesnitchenko V, Agius G, Zagury J F, Laaroubi K, Achour A, Castets M, Zagury D
Laboratoire de Physiologie Cellulaire, Université Pierre et Marie Curie, Paris, France.
J Med Microbiol. 1993 May;38(5):328-36. doi: 10.1099/00222615-38-5-328.
Serum and peripheral blood mononuclear cells from eight patients from the Ivory Coast with positive screening test results for retroviral infections were studied by serology (ELISA, Western blot (WB), synthetic peptide test), cell co-culture, and polymerase chain reaction (PCR). Two HIV-2 infections with indeterminate interpretation on HIV-1 WB were detected, two were clear dual HIV-1/HIV-2 infections, three were ambiguous mixed HIV-1/HIV-2 infections, and one was a triple retroviral infection by HTLV-I, HIV-1 and HIV-2. Four slow/low HIV-1 strains were isolated at the expense of HTLV-I and HIV-2 strains. The ELISA tests were found to be very sensitive. Indeterminate WB interpretations were frequent (HTLV-I, four; HIV-1, three; HIV-2, two). PCR provided clear evidence of multiple retroviral infections in three cases and enabled interpretation of indeterminate WB samples in three cases. One sample presented a puzzling pattern with positive PCR results for HIV-1 and HIV-2 associated with negative or indeterminate serological results. Thus, our data emphasise the need to analyse serological as well as virological markers to gain better insight on mixed retroviral infections, especially in endemic areas such as West Africa.
对来自科特迪瓦的8名筛查试验结果呈逆转录病毒感染阳性的患者的血清和外周血单核细胞进行了血清学（酶联免疫吸附测定、免疫印迹法（WB）、合成肽试验）、细胞共培养和聚合酶链反应（PCR）研究。检测到2例在HIV-1 WB检测结果不确定的HIV-2感染，2例为明确的HIV-1/HIV-2双重感染，3例为不明确的HIV-1/HIV-2混合感染，1例为HTLV-I、HIV-1和HIV-2的三重逆转录病毒感染。以牺牲HTLV-I和HIV-2毒株为代价分离出4株缓慢/低水平的HIV-1毒株。发现酶联免疫吸附测定试验非常敏感。免疫印迹法检测结果不确定的情况很常见（HTLV-I，4例；HIV-1，3例；HIV-2，2例）。PCR在3例中提供了多种逆转录病毒感染的明确证据，并在3例中能够解释免疫印迹法检测结果不确定的样本。1个样本呈现出令人困惑的模式，HIV-1和HIV-2的PCR结果为阳性，但血清学结果为阴性或不确定。因此，我们的数据强调了分析血清学和病毒学标志物以更好地了解混合逆转录病毒感染的必要性，特别是在西非等流行地区。
