Nakano Y, Kiyosawa K, Sodeyama T, Tanaka E, Yoshizawa K, Furuta K, Nakatsuji Y, Urushihara A, Furuta S
Second Dept. of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Scand J Gastroenterol. 1993 Apr;28(4):335-42. doi: 10.3109/00365529309090252.
To assess the role of anti-c100 antibodies to hepatitis C virus (anti-c100 HCV) in the response to interferon (IFN) administered to patients for the treatment of chronic hepatitis C, we assayed serum anti-c100 HCV serially by means of an enzyme-linked immunosorbent assay (ELISA) kit and titrated anti-c100 HCV level by a radioimmunoassay (RIA) kit in 28 IFN-treated and 20 untreated patients with chronic hepatitis C. IFN-alpha or -beta was administered for 4 to 12 weeks, and the patients were observed for over 24 months. The IFN-treated patients were divided into 3 groups (4 sustained responders, 18 transient responders, and 6 non-responders) in accordance with their responses on the basis of the serum alanine aminotransferase levels. In three of the four sustained responders whose HCV-RNA decreased, anti-c100 HCV became negative at 13, 15, and 17 months after beginning the IFN therapy. The 18 transient responders and 6 non-responders remained positive for anti-c100 HCV throughout the observation period. In all four sustained responders the anti-c100 HCV titer decreased significantly with time after initiation of the therapy, whereas the titer fluctuated in the other groups. These findings suggest that monitoring the serum anti-c100 HCV level is useful in assessing the effects of IFN therapy on chronic hepatitis C.
为评估抗丙型肝炎病毒c100抗体（抗-c100 HCV）在慢性丙型肝炎患者接受干扰素（IFN）治疗反应中的作用，我们采用酶联免疫吸附测定（ELISA）试剂盒对28例接受IFN治疗和20例未接受治疗的慢性丙型肝炎患者的血清抗-c100 HCV进行了连续检测，并使用放射免疫测定（RIA）试剂盒滴定抗-c100 HCV水平。给予α或β干扰素治疗4至12周，并对患者进行超过24个月的观察。根据血清丙氨酸氨基转移酶水平的反应情况，将接受IFN治疗的患者分为3组（4例持续应答者、18例短暂应答者和6例无应答者）。在4例HCV-RNA下降的持续应答者中，有3例在开始IFN治疗后13、15和17个月时抗-c100 HCV转为阴性。18例短暂应答者和6例无应答者在整个观察期内抗-c100 HCV均保持阳性。在所有4例持续应答者中，抗-c100 HCV滴度在治疗开始后随时间显著下降，而在其他组中滴度则有波动。这些发现表明，监测血清抗-c100 HCV水平有助于评估IFN治疗慢性丙型肝炎的效果。
