Garty H, Eisenbach M, Shuldman R, Caplan S R
Biochim Biophys Acta. 1979 Feb 8;545(2):365-75. doi: 10.1016/0005-2728(79)90213-5.
The kinetics of light-induced acidification and of the subsequent dark-induced alkalization in suspensions of sub-bacterial particles of Halobacterium halobium may be expressed as the sum of two exponentials, indicating two processes (Eisenbach, M., Bakker, E.P., Korenstein, R. and Caplan, S.R. (1976) FEBS Lett. 71, 228--232). We studied the effects of carbonyl cyanide p-trifluoromethyoxy phenyl-hydrazone, nigericin, gramicidin D, valinomycin, and monactin on the extents and the rate constants of the two processes. The various ionophores affected the two processes differently and in general the slower process was more sensitive to their presence. Valinomycin and monactin had relatively minor effects, apparently due to the high ionic strength of the suspension. When an artificial membrane potential was created in the dark, the light-induced acidification was preceded by a transient alkalization as is usually observed in intact cells. These results are discussed in the light of a suggested model accounting for the two processes (Caplan, S.R., Eisenbach, M., Cooper, S., Garty, H., Klemperer, G. and Bakker, E.P. (1977) in Bioenergetics of Membranes (Packer, L., Papageorgiou, G.C. and Trebst, A., eds.), pp. 101--114, Elsevier/North-Holland Biomedical Press, Amsterdam), taking into account the different selectivities of the ionophores applied.
嗜盐菌亚细菌颗粒悬浮液中光诱导酸化及随后暗诱导碱化的动力学可表示为两个指数之和，这表明存在两个过程（艾森巴赫，M.，巴克尔，E.P.，科伦斯坦，R.和卡普兰，S.R.（1976年）《欧洲生物化学学会联合会快报》71，228 - 232）。我们研究了羰基氰对 - 三氟甲氧基苯基腙、尼日利亚菌素、短杆菌肽D、缬氨霉素和莫能菌素对这两个过程的程度和速率常数的影响。各种离子载体对这两个过程的影响不同，一般来说，较慢的过程对它们的存在更敏感。缬氨霉素和莫能菌素的影响相对较小，显然是由于悬浮液的高离子强度。当在黑暗中产生人工膜电位时，光诱导酸化之前会出现短暂的碱化，这在完整细胞中通常可以观察到。根据一个解释这两个过程的建议模型（卡普兰，S.R.，艾森巴赫，M.，库珀，S.，加蒂，H.，克莱姆珀勒，G.和巴克尔，E.P.（1977年）载于《膜的生物能量学》（帕克，L.，帕帕佐格鲁，G.C.和特雷斯，A.编），第101 - 114页，爱思唯尔/北荷兰生物医学出版社，阿姆斯特丹），考虑到所应用离子载体的不同选择性，对这些结果进行了讨论。
