Sjöstrand N O, Beckett S D, Klinge E
Department of Physiology, Karolinska Institute, Stockholm, Sweden.
Acta Physiol Scand. 1993 Apr;147(4):403-15. doi: 10.1111/j.1748-1716.1993.tb09516.x.
The nervous control of the retractor penis muscle (rp) was investigated in the anaesthetized goat. Also, isolated field stimulated strips of the muscle were studied. The noradrenaline (NA) and acetylcholine (ACh) content of the rp was determined, and histochemistry for adrenergic and acetylcholinesterase (AChE) positive nerves was performed. The muscle exhibited spontaneous activity that persisted after section of all nerves. There was, however, also a tendency of the activity to follow the general vasomotor tone, which disappeared after section of the sympathetic chains. The excitatory adrenergic nerves which innervate the muscle come from the sympathetic chains and run along the pudendal, the hypogastric and the pelvic nerves. The rp has a dense network of adrenergic fibres and is very sensitive to excitatory adrenergic stimulation. It has a fairly large NA content, which is higher in old goats (5.95 +/- 0.42 micrograms g-1) than in young goats (2.87 +/- 0.78 micrograms g-1). Inhibitory non-adrenergic non-cholinergic (NANC) innervation reaches it via the pelvic and the hypogastric nerves. The maximum inhibitory response is reached at low frequencies (2-4 Hz). Cholinergic prejunctional inhibition of the excitatory response to sympathetic chain stimulation was effected by simultaneous stimulation of the hypogastric nerves. In vitro experiments confirmed the presence of endogenous cholinergic muscarinic suppression of the excitatory adrenergic neurotransmission. Significant amounts of ACh (0.81 +/- 0.18 micrograms g-1) are present in the muscle, and it contains strongly AChE positive nerve fibres and nerve cell bodies. It is concluded that the goat rp is innervated by sympathetic adrenergic excitatory nerves and parasympathetic NANC inhibitory nerves. It further has a direct sympathetic inhibitory NANC innervation, and an indirect inhibitory cholinergic innervation which at least in part is sympathetic.
在麻醉山羊身上研究了阴茎退缩肌（rp）的神经控制。此外，还对分离的该肌肉条进行了场刺激研究。测定了rp的去甲肾上腺素（NA）和乙酰胆碱（ACh）含量，并对肾上腺素能和乙酰胆碱酯酶（AChE）阳性神经进行了组织化学研究。该肌肉表现出在所有神经切断后仍持续存在的自发活动。然而，其活动也有跟随一般血管运动张力的趋势，在交感神经链切断后这种趋势消失。支配该肌肉的兴奋性肾上腺素能神经来自交感神经链，沿阴部神经、腹下神经和盆神经走行。rp有密集的肾上腺素能纤维网络，对兴奋性肾上腺素能刺激非常敏感。它的NA含量相当高，老年山羊（5.95±0.42微克/克）比幼年山羊（2.87±0.78微克/克）更高。抑制性非肾上腺素能非胆碱能（NANC）神经支配通过盆神经和腹下神经到达该肌肉。在低频（2 - 4Hz）时达到最大抑制反应。通过同时刺激腹下神经对交感神经链刺激的兴奋性反应产生胆碱能节前抑制。体外实验证实存在内源性胆碱能毒蕈碱对兴奋性肾上腺素能神经传递的抑制作用。肌肉中存在大量ACh（0.81±0.18微克/克），并且含有强AChE阳性神经纤维和神经细胞体。得出的结论是，山羊rp由交感肾上腺素能兴奋性神经和副交感NANC抑制性神经支配。它还具有直接的交感抑制性NANC神经支配以及间接的抑制性胆碱能神经支配，其中至少部分是交感神经的。
