Brown M, Bye A
J Chromatogr. 1977 Mar 1;143(2):195-202. doi: 10.1016/s0378-4347(00)81824-1.
A method is described for allopurinol and oxipurinol assay within human plasma and urine in the range expected during therapy. The method is based on high-performance ion-exchange chromatography following an efficient sample purification step using Chelex-100 resin in the Cu2+-form. Linear calibration curves are produced for allopurinol over the range 0.05-10 mumole/1 (0.068-1.36 mug/ml) in plasma and 0.005-1 mmole/1 (0.68-136 mug/ml) in urine and for oxipurinol 0.5-100 mumole/1 (0.076-15.2 mug/ml) in plasma and 0.1-2 mmole/1 (15.2-304 mug/ml) in urine.
本文描述了一种在治疗期间预期范围内测定人血浆和尿液中别嘌醇和氧嘌呤醇的方法。该方法基于高效离子交换色谱法，在使用Cu2+形式的Chelex-100树脂进行高效样品纯化步骤之后进行。在血浆中别嘌醇浓度范围为0.05 - 10微摩尔/升（0.068 - 1.36微克/毫升），尿液中为0.005 - 1毫摩尔/升（0.68 - 136微克/毫升）时可产生线性校准曲线；对于氧嘌呤醇，血浆中为0.5 - 100微摩尔/升（0.076 - 15.2微克/毫升），尿液中为0.1 - 2毫摩尔/升（15.2 - 304微克/毫升）时可产生线性校准曲线。
