Giron M L, Rozain F, Debons-Guillemin M C, Canivet M, Peries J, Emanoil-Ravier R
UPR A0043 CNRS Rétrovirus et Rétrotransposons des Vertébrés, Hôpital Saint-Louis, Paris, France.
J Virol. 1993 Jun;67(6):3596-600. doi: 10.1128/JVI.67.6.3596-3600.1993.
Human foamy virus (HFV) proteins were identified in human cells cultured in vitro by immunoprecipitation and immunoblotting with specific antisera. Among several viral polypeptides, four glycoproteins of approximately 160, 130, 70, and 48 kDa were identified in HFV-infected cells. gp130 was shown to represent the intracellular env precursor, and gp70 and gp48 were shown to represent the external and transmembrane env proteins, respectively. The nature of gp160, which shares sequences with the env, bel1, and bel2 proteins, is not yet resolved. In addition, a p62 identified with bel1- and bel2-specific antisera likely corresponds to the bet gene product.
通过免疫沉淀和使用特异性抗血清进行免疫印迹，在体外培养的人细胞中鉴定出了人泡沫病毒（HFV）蛋白。在几种病毒多肽中，在感染HFV的细胞中鉴定出了四种分子量约为160、130、70和48 kDa的糖蛋白。gp130被证明代表细胞内env前体，gp70和gp48分别被证明代表外部和跨膜env蛋白。与env、bel1和bel2蛋白具有相同序列的gp160的性质尚未明确。此外，用bel1和bel2特异性抗血清鉴定出的p62可能对应于bet基因产物。
