肌肉聚集蛋白：在神经诱导的乙酰胆碱受体簇处的神经调节与定位
Muscle agrin: neural regulation and localization at nerve-induced acetylcholine receptor clusters.
作者信息
Lieth E, Fallon J R
机构信息
Neurobiology Group, Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.
出版信息
J Neurosci. 1993 Jun;13(6):2509-14. doi: 10.1523/JNEUROSCI.13-06-02509.1993.
Abstract
The synapse-organizing protein agrin is expressed by muscle cells. In this study we begin to characterize the role of muscle agrin in synapse formation by investigating its distribution and expression during the formation of nerve-induced ACh receptor (AChR) aggregates in vitro. We have used species-specific anti-agrin antibodies to show that muscle agrin colocalizes with nerve-induced AChR clusters in chimeric nerve-muscle cocultures. Furthermore, quantitation by radioimmune assay shows that the expression of muscle agrin by cultured chick myotubes is increased more than twofold by coculture with cells from the motoneuron-rich ventral spinal cord. These data suggest a role for muscle agrin in neuromuscular junction development.
摘要
突触组织蛋白聚集蛋白由肌肉细胞表达。在本研究中，我们通过研究其在体外神经诱导的乙酰胆碱受体（AChR）聚集体形成过程中的分布和表达，开始对肌肉聚集蛋白在突触形成中的作用进行表征。我们使用种属特异性抗聚集蛋白抗体，以表明在嵌合神经-肌肉共培养物中，肌肉聚集蛋白与神经诱导的AChR簇共定位。此外，放射免疫测定法的定量分析表明，与富含运动神经元的脊髓腹侧细胞共培养时，培养的鸡肌管中肌肉聚集蛋白的表达增加了两倍多。这些数据表明肌肉聚集蛋白在神经肌肉接头发育中起作用。
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