放射性标记的抗血管紧张素转换酶单克隆抗体的纯化显著提高了其靶向肺部的特异性和有效性。

Purification of radiolabeled monoclonal antibodies to angiotensin-converting enzyme significantly improves specificity and efficacy of its targeting into the lung.

作者信息

Hiemisch H, Gavrilyuk V, Atochina E, Slinkin M, Torchilin V, Muzykantov V, Danilov S

机构信息

National Cardiology Research Center, Moscow, Russia.

出版信息

Nucl Med Biol. 1993 May;20(4):435-41. doi: 10.1016/0969-8051(93)90074-5.

DOI:10.1016/0969-8051(93)90074-5

PMID:8389221

Abstract

The aim of this study was to improve the labeling/purification procedures for monoclonal antibody (MoAb) to angiotensin-converting enzyme (ACE). MoAb 9B9 was very stable upon iodination at a wide range of iodogen concentrations and incubation times, and was also very stable upon storage, indicating the high technological potential of this MoAb. Radiolabeled MoAb 9B9 was purified by (i) adsorption chromatography on cellulose, (ii) HPLC (gel filtration) and (iii) affinity chromatography on ACE-Sepharose. The best result was obtained with cellulose: specificity of MoAb 9B9 accumulation in the lung increased 2-fold. We conclude that the phenomenon of specific lung accumulation of MoAb 9B9 may serve as an ideal (convenient, cheap and technological) assay system for evaluation of monoclonal antibody modification and labeling.

摘要

本研究的目的是改进针对血管紧张素转换酶（ACE）的单克隆抗体（MoAb）的标记/纯化程序。单克隆抗体9B9在广泛的碘甘醚浓度和孵育时间下进行碘化时非常稳定，并且在储存时也非常稳定，这表明该单克隆抗体具有很高的技术潜力。放射性标记的单克隆抗体9B9通过以下方法纯化：（i）在纤维素上进行吸附色谱法，（ii）高效液相色谱法（凝胶过滤），以及（iii）在ACE-琼脂糖上进行亲和色谱法。使用纤维素获得了最佳结果：单克隆抗体9B9在肺中积累的特异性提高了2倍。我们得出结论，单克隆抗体9B9在肺中特异性积累的现象可作为评估单克隆抗体修饰和标记的理想（方便、廉价且技术可行）检测系统。