West J R, Nornes H O, Barnes C L, Bronfenbrenner M
Brain Res. 1979 Jan 12;160(2):203-15. doi: 10.1016/0006-8993(79)90419-0.
The hippocampal commissural projection to the area dentata of the mouse was studied using the retrograde horseradish peroxidase (HRP) technique. Small volumes of HRP injected into the molecular layer of the fascia dentata or various subareas of regio inferior of the hippocampus (fields CA3a-c) resulted inlabeled perikarya in the contralateral hippocampus and area dentata. The commissural projection to the fascia dentata was observed to originate exclusively from cells within the hilus fasciae dentatae (CA4) of the contralateral area dentata. There was evidence of a considerable spread of commissural innervation along the septotemporal axis preferentially in the septal direction, confirming earlier observations. In contrast to the septotemporal spread, a sharp homotopic spatial organization was found in the mediolateral direction. For example, injections into the lateral portion of field CA3 (CA3a) resulted in HRP-positive cell bodies only in the contralateral field CA3a. When injections were made which apparently labeled all of the commissural fibers, the HRP reaction product was found in neurons both in the entire regio inferior and as far as the innermost point of the hilus fasciae dentatae; the majority of labeled cells were located in hippocampal subfield CA3c. No labeled cells were observed beyond the tip of the mossy fibers in regio superior.
采用逆行辣根过氧化物酶（HRP）技术，对小鼠海马连合投射至齿状回区域进行了研究。将少量HRP注入齿状回分子层或海马下区（CA3a - c区）的各个亚区，结果在对侧海马和齿状回中发现了标记的核周体。观察到连合投射至齿状回的纤维仅起源于对侧齿状回齿状筋膜（CA4）内的细胞。有证据表明，连合神经支配沿颞中隔轴有相当程度的扩散，且优先向中隔方向扩散，这证实了早期的观察结果。与颞中隔方向的扩散不同，在内外侧方向发现了明显的同位空间组织。例如，向CA3区（CA3a）外侧部分注射后，仅在对侧CA3a区发现HRP阳性细胞体。当进行的注射明显标记了所有连合纤维时，在整个海马下区以及齿状筋膜最内侧点的神经元中均发现了HRP反应产物；大多数标记细胞位于海马CA3c亚区。在海马上部苔藓纤维末端以外未观察到标记细胞。
