Sigel E, Baur R, Malherbe P
Institute of Pharmacology, University of Bern, Switzerland.
FEBS Lett. 1993 Jun 14;324(2):140-2. doi: 10.1016/0014-5793(93)81380-i.
Different combinations of cloned subunits of the rat brain GABAA receptor were expressed in Xenopus oocytes. Possible effects of ethanol on the expressed GABA-induced chloride current were determined. The consequence of replacing the gamma 2S subunit by the alternatively spliced variant gamma 2L was specifically tested on the responsiveness to ethanol. A significant stimulation of the GABA response was only observed at very high concentrations (> 60 mM) of ethanol. No differential response was observed between subunit combinations containing different gamma 2 subunit splice variants.
大鼠脑γ-氨基丁酸A（GABAA）受体的克隆亚基的不同组合在非洲爪蟾卵母细胞中表达。测定了乙醇对所表达的γ-氨基丁酸诱导的氯离子电流的可能影响。具体测试了用可变剪接变体γ2L替代γ2S亚基对乙醇反应性的影响。仅在非常高浓度（>60 mM）的乙醇下才观察到γ-氨基丁酸反应的显著刺激。在含有不同γ2亚基剪接变体的亚基组合之间未观察到差异反应。
