Woodruff W H
Spectroscopy and Biochemistry Group, Los Alamos National Laboratory, New Mexico 87545.
J Bioenerg Biomembr. 1993 Apr;25(2):177-88. doi: 10.1007/BF00762859.
Results are presented which, taken with evidence developed by others, suggest a general mechanism for the entry and binding of exogenous ligands (including O2) at the "binuclear site" (CuBFea3) of the heme-copper oxidases. The mechanism includes a "ligand shuttle" wherein the obligatory way station for incoming ligands is CuB and the binding of exogenous ligands at this site triggers the exchange and displacement of endogenous ligands at Fea3. It is suggested that these ligand shuttle reactions might be functionally important in providing a coupling mechanism for electron transfer and proton translocation. Scenarios as to how this might happen are delineated.
研究结果表明，结合其他研究得出的证据，可提出一种关于外源性配体（包括O2）在血红素-铜氧化酶的“双核位点”（CuBFea3）进入和结合的通用机制。该机制包括一个“配体穿梭”过程，即进入的配体的必经站点是CuB，外源性配体在此位点的结合会触发Fea3上内源性配体的交换和置换。有人认为，这些配体穿梭反应可能在为电子转移和质子转运提供偶联机制方面具有重要功能。文中描述了这种情况可能发生的几种设想。
