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人乳头瘤病毒18型基因组中新型启动子的鉴定与特征分析

Identification and characterization of novel promoters in the genome of human papillomavirus type 18.

作者信息

Karlen S, Beard P

机构信息

Department of Virology, Swiss Institute for Experimental Cancer Research, Epalinges.

出版信息

J Virol. 1993 Jul;67(7):4296-306. doi: 10.1128/JVI.67.7.4296-4306.1993.

DOI:10.1128/JVI.67.7.4296-4306.1993
PMID:8389929
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC237800/
Abstract

Most studies on the regulation of gene expression in human papillomaviruses (HPV) have focused on the promoter for the early genes E6 and E7. This promoter is located at the junction between the long control region and the E6 open reading frame. RNA mapping studies have suggested that additional promoters may exist in other parts of the genome. In this study, we used a combination of transcription in vitro and an analysis of RNA produced in vivo in transfected cells to identify three novel promoters in the genome of human papillomavirus type 18. These promoters are located in front of the E2 gene (P2598), within the E2 coding sequences (P3036), and at the end of the L2 open reading frame (P5600). They were active in HeLa cells, as shown by a chloramphenicol acetyltransferase assay. The activity of the P3036 promoter was stimulated by the bovine papillomavirus type 1 E2 protein.

摘要

大多数关于人乳头瘤病毒（HPV）基因表达调控的研究都集中在早期基因E6和E7的启动子上。该启动子位于长控制区与E6开放阅读框的交界处。RNA图谱研究表明，基因组的其他区域可能存在额外的启动子。在本研究中，我们结合体外转录和对转染细胞体内产生的RNA的分析，在18型人乳头瘤病毒基因组中鉴定出三个新的启动子。这些启动子位于E2基因之前（P2598）、E2编码序列内（P3036）以及L2开放阅读框末端（P5600）。如氯霉素乙酰转移酶测定所示，它们在HeLa细胞中具有活性。P3036启动子的活性受到1型牛乳头瘤病毒E2蛋白的刺激。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/826e83b00793/jvirol00028-0616-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/9ed940d50f7f/jvirol00028-0612-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/8a321c578d81/jvirol00028-0613-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/a2f2b45ebf96/jvirol00028-0613-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/b0babe5fcf3e/jvirol00028-0614-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/e70902f9ec9a/jvirol00028-0614-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/b7efb526ee2d/jvirol00028-0615-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/8bacf9ac5a52/jvirol00028-0615-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/826e83b00793/jvirol00028-0616-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/9ed940d50f7f/jvirol00028-0612-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/8a321c578d81/jvirol00028-0613-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/a2f2b45ebf96/jvirol00028-0613-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/b0babe5fcf3e/jvirol00028-0614-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/e70902f9ec9a/jvirol00028-0614-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/b7efb526ee2d/jvirol00028-0615-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/8bacf9ac5a52/jvirol00028-0615-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97eb/237800/826e83b00793/jvirol00028-0616-a.jpg

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