Stemler A
Department of Botany, University of California, Davis 95616.
Anal Biochem. 1993 May 1;210(2):328-31. doi: 10.1006/abio.1993.1203.
An assay procedure for carbonic anhydrase activity is described. Unique to this method is the use of radiolabeled bicarbonate and a reaction vessel with a gas-permeable membrane. The method detects 14CO2 production directly and can be used at room temperature and with solutions buffered over a wide pH range. Assays are rapid (5-10 s), discounting elution and scintillation counting time. The equipment required is simple and inexpensive, marking the method ideal for routine determinations of carbonic anhydrase activity. The method can also be applied to other reactions that liberate radioactive CO2, other labeled gases, or small unchanged, labeled molecules.
本文描述了一种碳酸酐酶活性的测定方法。该方法的独特之处在于使用了放射性标记的碳酸氢盐和带有透气膜的反应容器。该方法可直接检测14CO2的生成，并且可在室温下以及在较宽pH范围内缓冲的溶液中使用。测定快速（5 - 10秒），不包括洗脱和闪烁计数时间。所需设备简单且价格低廉，这使得该方法成为碳酸酐酶活性常规测定的理想选择。该方法还可应用于其他释放放射性CO2、其他标记气体或未改变的小标记分子的反应。
