Sah P, Francis K, McLachlan E M, Junankar P
Department of Physiology and Pharmacology, University of Queensland, Australia.
Neuroscience. 1993 May;54(1):157-65. doi: 10.1016/0306-4522(93)90391-r.
The distributions of ryanodine receptor-like immunoreactivity and Ca-ATPase-like immunoreactivity were identified in the guinea-pig and rat central nervous system using antibodies raised against the rabbit skeletal muscle ryanodine receptor and Ca-ATPase. In both guinea-pig and rat cerebellum, the ryanodine receptor-like immunoreactivity was restricted to the soma and dendrites of Purkinje cells. In the medulla, neuron somata in the hypoglossal nucleus were stained in both species, but in the dorsal motor nucleus of the vagus somata were stained in guinea-pigs but not in rats. This species difference in ryanodine receptor-like immunoreactivity is consistent with the species difference in expression of a ryanodine sensitive, calcium activated potassium conductance in neurons of the dorsal motor nucleus of the vagus. Immunoreactivity to Ca-ATPase was present in vagal motoneurons in both species with denser staining in the guinea-pig. The data further support the idea that, in neurons of the dorsal motor nucleus of the vagus, release of intracellular calcium stores via a ryanodine receptor activates a specific class of potassium channels, thereby modulating cell excitability.
利用针对兔骨骼肌兰尼碱受体和钙 -ATP 酶产生的抗体，在豚鼠和大鼠中枢神经系统中鉴定了兰尼碱受体样免疫反应性和钙 -ATP 酶样免疫反应性的分布。在豚鼠和大鼠的小脑中，兰尼碱受体样免疫反应性仅限于浦肯野细胞的胞体和树突。在延髓中，舌下神经核的神经元胞体在两种动物中均被染色，但在迷走神经背运动核中，豚鼠的胞体被染色，而大鼠的未被染色。迷走神经背运动核中兰尼碱受体样免疫反应性的这种物种差异与迷走神经背运动核神经元中兰尼碱敏感的钙激活钾电导表达的物种差异一致。两种动物的迷走运动神经元中均存在对钙 -ATP 酶的免疫反应性，豚鼠中的染色更密集。这些数据进一步支持了这样一种观点，即在迷走神经背运动核的神经元中，通过兰尼碱受体释放细胞内钙储存会激活一类特定的钾通道，从而调节细胞兴奋性。
