Thomson D M
Cancer Res. 1979 Feb;39(2 Pt 2):627-9.
Heparinized samples of blood from three different patients were coded by impartial observers. The buffy coat leukocytes from the coded samples of blood were isolated and incubated separately with extracts of colon and pancreatic cancer in the tube leukocyte adherence inhibition assay. At the completion of the assay, the leukocytes from Patient 1 were equally nonadherent to both cancer extracts with a nonadherence index value of 8. By contrast, leukocytes from Patient 2 exhibited increased nonadherence to the extract of colon cancer (p = 0.02) with a nonadherence index value to colon cancer antigen of 89. Leukocytes from Patient 3 displayed increased nonadherence to the extract of pancreatic cancer (p less than 0.05) with a nonadherence index value to pancreatic cancer antigen of 39. When the code was broken, patients 1, 2, and 3 had diagnoses of malignant melanoma, colon cancer, and pancreatic cancer, respectively. Hence, this was a classical criss-cross experiment; the patient with malignant melanoma reacted to neither of the antigens, whereas the patients with colon and pancreatic cancer reacted to the sensitizing cancers which had unique organ-type specific neoantigens.
来自三名不同患者的肝素化血液样本由公正的观察者进行编码。在试管白细胞黏附抑制试验中，对编码后的血液样本中的血沉棕黄层白细胞进行分离，并分别与结肠癌和胰腺癌提取物一起孵育。试验结束时，患者1的白细胞对两种癌症提取物的非黏附性相同，非黏附指数值为8。相比之下，患者2的白细胞对结肠癌提取物的非黏附性增加（p = 0.02），对结肠癌抗原的非黏附指数值为89。患者3的白细胞对胰腺癌提取物的非黏附性增加（p小于0.05），对胰腺癌抗原的非黏附指数值为39。当编码被破解时，患者1、2和3分别被诊断为恶性黑色素瘤、结肠癌和胰腺癌。因此，这是一个典型的交叉试验；患有恶性黑色素瘤的患者对两种抗原均无反应，而患有结肠癌和胰腺癌的患者对具有独特器官类型特异性新抗原的致敏癌症有反应。
