Pyke C, Eriksen J, Solberg H, Nielsen B S, Kristensen P, Lund L R, Danø K
Finsen Laboratory, Rigshospitalet, Copenhagen, Denmark.
FEBS Lett. 1993 Jul 12;326(1-3):69-74. doi: 10.1016/0014-5793(93)81763-p.
Using 3' RACE (rapid amplification of cDNA ends), we have isolated a cDNA variant for the receptor for human urokinase plasminogen activator (uPAR). The deduced protein includes the amino-terminal ligand binding domain in uPAR, but lacks the carboxy-terminal membrane attachment by a glycolipid anchor. Genomic DNA analysis showed that the uPAR mRNA variant is generated by alternative splicing. The new variant mRNA is expressed in various human cell lines and tissues and both variants are up-regulated by phorbol ester in A549 cells. We propose that the alternatively spliced uPAR mRNA encodes a soluble uPA binding protein, the possible function of which is discussed.
利用3' RACE（cDNA末端快速扩增）技术，我们分离出了人尿激酶型纤溶酶原激活剂（uPAR）受体的一种cDNA变体。推导的蛋白质包含uPAR中氨基末端的配体结合结构域，但缺少通过糖脂锚定的羧基末端膜附着结构域。基因组DNA分析表明，uPAR mRNA变体是通过可变剪接产生的。新的变体mRNA在多种人类细胞系和组织中表达，并且在A549细胞中，两种变体均被佛波酯上调。我们提出，可变剪接的uPAR mRNA编码一种可溶性uPA结合蛋白，并对其可能的功能进行了讨论。
