Bell K L, Smith V J
School of Biology and Medical Sciences, Gatty Marine Laboratory, University of St. Andrews, Fife, Scotland.
Dev Comp Immunol. 1993 May-Jun;17(3):211-9. doi: 10.1016/0145-305x(93)90040-w.
This study examines the phagocytic hyaline cells of the shore crab, Carcinus maenas, for a respiratory burst in vitro. Following stimulation of the cells with phorbol myristate acetate (PMA), ferricytochrome c was reduced at 550 nm. Addition of superoxide dismutase (SOD) to the reaction mixture decreased this reduction, confirming that superoxide ions are produced by the stimulated hemocytes. Phytohemagglutinin, con A, and LPS were also shown to stimulate the cells although laminarin failed to elicit a burst. This is the first demonstration of a respiratory burst for crustacean hemocytes.
本研究检测了海滨蟹（Carcinus maenas）的吞噬性透明细胞，以观察其体外呼吸爆发情况。在用佛波酯（PMA）刺激细胞后，高铁细胞色素c在550nm处被还原。向反应混合物中添加超氧化物歧化酶（SOD）可减少这种还原，证实受刺激的血细胞会产生超氧阴离子。植物血凝素、刀豆蛋白A和脂多糖也被证明能刺激细胞，尽管海带多糖未能引发呼吸爆发。这是首次证明甲壳类血细胞存在呼吸爆发。
