Bishop A, Paz M A, Gallop P M, Karnovsky M L
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Free Radic Biol Med. 1995 Mar;18(3):617-20. doi: 10.1016/0891-5849(94)00178-m.
The iodonium compounds diphenyleneiodonium and diphenyliodonium, and the amine compounds, 4,5-dimethyl phenylene diamine, N,N-dimethyl 1,4-phenylene diamine, 1,2-diamino-4,5-methyleneoxybenzene, and aminomalononitrile inhibit methoxatin's (PQQ's) redox activity in vitro, that is, the methoxatin-coupled oxidation of glycine and reduction of nitroblue tetrazolium to formazan. The compounds mentioned above also inhibit phorbol myristate acetate (PMA) stimulated superoxide release by phagocytic white cells--determined mainly as the superoxide dismutase sensitive reduction of ferricytochrome C. Related compounds, 3,4-diaminopyridine and 4-dimethylamino-benzylamine, did not inhibit redox activity of PQQ in vitro, nor did they inhibit PMA stimulated superoxide production in monocytes or neutrophils. Thus, there is a correlation between an agent's ability to inhibit PQQ redox cycling and its ability to inhibit superoxide release by phagocytes. The findings are a further indication that PQQ is involved in the respiratory burst of phagocytic cells.
碘鎓化合物二亚苯基碘鎓和二苯基碘鎓，以及胺类化合物4,5-二甲基对苯二胺、N,N-二甲基-1,4-对苯二胺、1,2-二氨基-4,5-亚甲氧基苯和氨基丙二腈在体外抑制甲氧苄氨嘧啶（PQQ）的氧化还原活性，即甲氧苄氨嘧啶偶联的甘氨酸氧化和硝基蓝四唑还原为甲臜。上述化合物还抑制佛波酯（PMA）刺激的吞噬白细胞释放超氧化物——主要通过超氧化物歧化酶敏感的高铁细胞色素C还原来测定。相关化合物3,4-二氨基吡啶和4-二甲基氨基苄胺在体外不抑制PQQ的氧化还原活性，也不抑制PMA刺激的单核细胞或中性粒细胞产生超氧化物。因此，一种试剂抑制PQQ氧化还原循环的能力与其抑制吞噬细胞释放超氧化物的能力之间存在相关性。这些发现进一步表明PQQ参与吞噬细胞的呼吸爆发。
