Carroll W L
Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St Louis.
Am J Clin Nutr. 1993 Aug;58(2 Suppl):249S-258S. doi: 10.1093/ajcn/58.2.249S.
Recombinant-DNA technology is now commonly used in virtually every aspect of the biological sciences. The purpose of this brief exposition is to provide an outline of the approaches used to identify genes, to isolate the gene of interest, to amplify the gene if necessary, and to clone genes. A short introduction to the principles of separating very large genes is provided, along with a description of an approach to propagating and cloning these large genes.
重组DNA技术如今实际上已普遍应用于生物科学的各个方面。本简要阐述的目的是概述用于识别基因、分离感兴趣的基因、必要时扩增基因以及克隆基因的方法。还提供了关于分离非常大的基因的原理的简短介绍，以及对这些大基因进行繁殖和克隆的方法的描述。
