Wilson A C, LaMarco K, Peterson M G, Herr W
Cold Spring Harbor Laboratory, New York 11724.
Cell. 1993 Jul 16;74(1):115-25. doi: 10.1016/0092-8674(93)90299-6.
Upon lytic infection of permissive cells, the herpes simplex virus (HSV) transactivator protein VP16 associates with an accessory protein termed host cell factor (HCF). Binding to HCF activates VP16 for association with the octamer motif-binding protein Oct-1, to form a multiprotein-DNA complex responsible for activating transcription of the HSV immediate early genes. We show that HCF comprises a series of related polypeptides that range from 110 to 300 kd, all of which are encoded by a single gene. Although there is no obvious sequence similarity between HCF and other known proteins, HCF contains eight repeats of a new 26 amino acid motif. cDNAs encoding HCF predict a large open reading frame of 2035 codons. When expressed in human cells, this large open reading frame encodes both the 300 kd and smaller HCF polypeptides, indicating that the smaller polypeptides arise by processing of the 300 kd protein.
在对允许性细胞进行裂解感染时，单纯疱疹病毒（HSV）反式激活蛋白VP16与一种称为宿主细胞因子（HCF）的辅助蛋白结合。与HCF结合可激活VP16，使其与八聚体基序结合蛋白Oct-1结合，形成一种负责激活HSV立即早期基因转录的多蛋白-DNA复合物。我们发现HCF由一系列分子量在110至300kd之间的相关多肽组成，所有这些多肽均由单个基因编码。尽管HCF与其他已知蛋白之间没有明显的序列相似性，但HCF含有一个新的26个氨基酸基序的八个重复序列。编码HCF的cDNA预测有一个2035个密码子的大开放阅读框。当在人类细胞中表达时，这个大开放阅读框编码300kd的HCF多肽以及较小的HCF多肽，这表明较小的多肽是由300kd蛋白加工产生的。
