Perraut R, Hundt E, Garraud O, Enders B, Gysin J
Laboratoire d'Immunologie Parasitaire, Institut Pasteur de la Guyane Francaise, Cayenne.
Vaccine. 1993;11(7):730-6. doi: 10.1016/0264-410x(93)90257-x.
The effect of several adjuvants and of adjuvant doses on the quantitative and qualitative antibody response to tetanus toxoid (TT) and a recombinant herpes simplex virus peptide (HSVgD) was evaluated in the New World monkey Saimiri sciureus. All adjuvant formulations were effective in inducing a strong antibody response to these antigens. The qualitative antibody response, as defined by monoclonal antibodies 3A2/G6 and 4G3/B5, was determined. Only 3A2/G6+ antibodies were induced after immunization with HSVgD irrespective of the adjuvant used and after immunization with TT alone. However, both types of antibodies were induced against TT in the presence of the adjuvants tested. These data suggest that both adjuvant and antigens can intervene in driving the quality of the antibody response induced after immunization. Strong antibody response to TT was induced at any adjuvant dose tested, suggesting that lower doses are as effective as higher doses. These findings can have implications on the design of future trials of vaccine candidate constructs in monkeys and eventually in human beings.
在新大陆猴松鼠猴（Saimiri sciureus）中评估了几种佐剂及其剂量对破伤风类毒素（TT）和重组单纯疱疹病毒肽（HSVgD）的定量和定性抗体反应的影响。所有佐剂配方均能有效诱导针对这些抗原的强烈抗体反应。测定了由单克隆抗体3A2/G6和4G3/B5定义的定性抗体反应。无论使用何种佐剂，用HSVgD免疫后以及仅用TT免疫后，仅诱导出3A2/G6+抗体。然而，在测试的佐剂存在下，针对TT诱导出了两种类型的抗体。这些数据表明，佐剂和抗原均可干预免疫后诱导的抗体反应的质量。在任何测试的佐剂剂量下均诱导出对TT的强烈抗体反应，表明较低剂量与较高剂量一样有效。这些发现可能对未来在猴中以及最终在人类中进行候选疫苗构建体试验的设计具有启示意义。
