人精浆磷酸酶。特性及其与植物磷酸酶的比较。

Human seminal phosphatase. Properties and comparison with plant phosphatases.

Fujisawa K, Katsumata Y, Yoshino M

Department of Legal Medicine, Nagoya University School of Medicine, Japan.

Int J Biochem. 1993 May;25(5):627-30. doi: 10.1016/0020-711x(93)90345-f.

Abstract

Phosphatase activities were determined in various seminal fluid and plant tissues. 2. Human semen showed a markedly higher phosphatase activity, and some plants and mushrooms exhibited a considerably higher phosphatase activity. 3. Phosphatase purified from human seminal fluid showed a pH optimum of 5-6 and was potently inhibited by L-(+)-tartrate. 4. Tartrate could not inhibit most plant phosphatases, but inhibited the mushroom phosphatase with a one-order lower affinity than that of the seminal enzyme.

摘要

测定了各种精液和植物组织中的磷酸酶活性。2. 人类精液显示出明显更高的磷酸酶活性，一些植物和蘑菇表现出相当高的磷酸酶活性。3. 从人类精液中纯化的磷酸酶的最适pH值为5 - 6，并且受到L-(+)-酒石酸的强烈抑制。4. 酒石酸不能抑制大多数植物磷酸酶，但对蘑菇磷酸酶的抑制亲和力比精液中的酶低一个数量级。