Mitin Y V, Navolotskaya E V, Vasilenko R N, Abramov V M, Zav'Yalov V P
Institute of Immunology, Biopreparation State Concern, Moscow Region, Russia.
Int J Pept Protein Res. 1993 Jun;41(6):517-21. doi: 10.1111/j.1399-3011.1993.tb00472.x.
The decapeptide Val-Lys-Lys-Pro-Gly--Ser-Ser-Val-Lys-Val (termed immunocorticotropin) corresponding to the sequence 11-20 of the variable part of the human immunoglobulin G1 heavy chain was synthesized. The ACTH-like peptide was found to have an ability to increase body temperature. Intracerebroventricular injection of 5-10 micrograms of the peptide to rabbits induced an elevation of body temperature by 0.7-1.3 degrees C. The ACTH-like peptide was shown to compete with [125I]-ACTH (13-24) for binding to receptors on murine brain synaptic membranes and to activate adenylate cyclase of these membranes.
合成了与人类免疫球蛋白G1重链可变部分序列11 - 20相对应的十肽Val - Lys - Lys - Pro - Gly - Ser - Ser - Val - Lys - Val（称为免疫促肾上腺皮质激素）。发现这种促肾上腺皮质激素样肽具有升高体温的能力。向兔子脑室内注射5 - 10微克该肽可使体温升高0.7 - 1.3摄氏度。该促肾上腺皮质激素样肽显示出能与[125I] - ACTH（13 - 24）竞争结合鼠脑突触膜上的受体，并激活这些膜的腺苷酸环化酶。
