Beketić-Oresković L, Sarcević B, Malenica B, Novak D
Ruder Bosković Institute, Zagreb, Croatia.
Neoplasma. 1993;40(2):69-74.
Our previous report revealed the production of monoclonal antibody (MoAb) CDI 315B by immunization of mice with tumor extract proteins of human invasive ductal breast carcinoma. In the present study we report on the immunocytochemical reactivity of this MoAb with formalin or methacarn fixed, paraffin embedded tissue sections and also with cell cultures. Among breast tissues, positive staining was detected in 88% (64 of 73) of primary breast carcinomas, 77% (7 of 9) of metastatic lymph nodes, 24% (8 of 33) of benign breast disease and 15% (2 of 13) of normal breast tissue. No immunostaining was detected with several other tumors, with the exception of melanoma, where 63% (5 of 8) of positive staining was found. On in vitro cell lines, positive reaction was detected only with breast carcinoma and melanoma cells, but not with other examined cell lines. On benign breast disease tissue sections, positive reaction was detected in areas with cell hyperplasia. On normal breast tissue sections MoAb 315B stained the epithelial cells of terminal ductuli. Since the MoAb 315B recognized some antigen present in the cytoplasm of most breast carcinoma cells, this MoAb may have potential application in diagnosis and management of breast cancer.
我们之前的报告显示，通过用人浸润性导管乳腺癌的肿瘤提取物蛋白免疫小鼠可产生单克隆抗体（MoAb）CDI 315B。在本研究中，我们报告了该单克隆抗体与福尔马林或甲醇固定、石蜡包埋的组织切片以及细胞培养物的免疫细胞化学反应性。在乳腺组织中，88%（73例中的64例）的原发性乳腺癌、77%（9例中的7例）的转移性淋巴结、24%（33例中的8例）的良性乳腺疾病以及15%（13例中的2例）的正常乳腺组织检测到阳性染色。除黑色素瘤外，其他几种肿瘤均未检测到免疫染色，黑色素瘤中63%（8例中的5例）呈阳性染色。在体外细胞系中，仅在乳腺癌和黑色素瘤细胞中检测到阳性反应，而在其他检测的细胞系中未检测到。在良性乳腺疾病组织切片中，在细胞增生区域检测到阳性反应。在正常乳腺组织切片上，单克隆抗体315B对终末小导管的上皮细胞进行染色。由于单克隆抗体315B识别大多数乳腺癌细胞质中存在的某些抗原，该单克隆抗体可能在乳腺癌的诊断和治疗中具有潜在应用价值。
