Zagorodnyuk V P, Hoyle C H, Burnstock G
Department of Anatomy and Developmental Biology, University College London, UK.
Pflugers Arch. 1993 Jun;423(5-6):427-33. doi: 10.1007/BF00374937.
A sucrose-gap technique was used to study the development of neuromuscular transmission in the taenia coli of fetal, 1- to 2-day-old, 3- to 4-week-old and 3-month-old guinea-pigs. In addition, the effects of exogenous, alpha,beta-methylene adenosine 5'-triphosphate (ATP), noradrenaline, vasoactive intestinal polypeptide (VIP) and carbachol were examined. Taking the response to a single pulse of electrical field stimulation as the index of a developed neuromuscular junction, it was apparent that the non-adrenergic inhibitory system arose before, and matured more quickly than, the cholinergic system. The inhibitory system was present by 8 weeks of gestation in some fetuses, but, in contrast, excitatory cholinergic transmission was not seen until birth. As evidenced by responses to carbachol, alpha,beta-methylene ATP and VIP, cholinergic, purinergic and VIP receptors were present on the smooth muscle at the earliest ages studied. No changes in sensitivity to these agents were noted throughout development, although in adults the level of the maximum responses increased.
采用蔗糖间隙技术研究了胎儿、1至2日龄、3至4周龄和3月龄豚鼠结肠带神经肌肉传递的发育情况。此外，还检测了外源性α,β-亚甲基腺苷5'-三磷酸（ATP）、去甲肾上腺素、血管活性肠肽（VIP）和卡巴胆碱的作用。以对单个电场刺激脉冲的反应作为成熟神经肌肉接头的指标，很明显，非肾上腺素能抑制系统比胆碱能系统出现得早且成熟得更快。在一些胎儿中，抑制系统在妊娠8周时就已存在，但相比之下，兴奋性胆碱能传递直到出生时才出现。在所研究的最早年龄阶段，平滑肌上就存在胆碱能、嘌呤能和VIP受体，这可通过对卡巴胆碱、α,β-亚甲基ATP和VIP的反应得到证明。在整个发育过程中，对这些药物的敏感性没有变化，尽管在成年动物中最大反应水平有所增加。
