Pavelic K, Banjac Z, Pavelic J, Spaventi S
Department of Molecular Medicine, Ruder Boskovic Institute, Zagreb, Croatia.
Anticancer Res. 1993 Jul-Aug;13(4):1133-7.
Sixty-three primary lung carcinomas were examined immunohistochemically for the presence of epidermal growth factor receptors (EGF-R). Of these tumors 35 (55.5%) were positive for EGF-R. A positive correlation between overexpression of EGF-R, on the one hand, and high metastatic rate, poor tumor differentiation and high rate of tumor proliferation, on the other hand, was found. From one tumor with an extremely high amount of EGF-R a primary cell culture was established. The addition of anti-EGF-R serum to this culture decreased cell proliferation. Our results support the evidence for a positive relationship between overexpression of EGF-R and invasiveness, poor differentiation and high growth rate of tumor cells. The blockade of EGF-R with specific antibodies suppressed tumor cell proliferation, suggesting a role of EGF-R in tumor progression.
对63例原发性肺癌进行免疫组织化学检查，以检测表皮生长因子受体（EGF-R）的存在情况。在这些肿瘤中，35例（55.5%）EGF-R呈阳性。研究发现，一方面EGF-R的过表达与另一方面的高转移率、肿瘤低分化及高肿瘤增殖率之间存在正相关。从一例EGF-R含量极高的肿瘤中建立了原代细胞培养。向该培养物中添加抗EGF-R血清可降低细胞增殖。我们的结果支持了EGF-R过表达与肿瘤细胞侵袭性、低分化及高生长率之间存在正相关的证据。用特异性抗体阻断EGF-R可抑制肿瘤细胞增殖，提示EGF-R在肿瘤进展中起作用。
