Hasslen S R, Nelson R D, Ahrenholz D H, Solem L D
Burn Center, St. Paul-Ramsey Medical Center, Minnesota.
J Burn Care Rehabil. 1993 May-Jun;14(3):303-9. doi: 10.1097/00004630-199305000-00001.
The purpose of this study was to assess the influence of thermal injury and the inflammatory process on chemotactic responses of neutrophils to four attractants (N-formyl-methionyl-leucyl-phenylalanine, the complement fragment C5a, interleukin-8, and leukotriene B4) under agarose, expression of Mac-1 (CD11b/CD18) adherence receptors on the cell surface, and polymerization of actin in the cell cytoplasm. Circulating neutrophils were isolated from peripheral blood, and exudate neutrophils from fluid collecting under two different wound dressings applied to abrasion sites of healthy subjects and to skin graft donor sites of patients with burns. Burn injury reduced the chemotactic responses of circulating neutrophils to all four attractants, suggesting a "global" defect in chemotactic function. Patient-exudate neutrophils collected under Tegaderm exhibited further decrements in all chemotactic responses, and patient-exudate neutrophils collected under Biobrane were nonmotile. The exudate neutrophils collected under Biobrane expressed high levels of Mac-1 receptors and irreversibly polymerized actin, which may contribute to the nonmotility of these exudate cells.
本研究的目的是评估热损伤和炎症过程对中性粒细胞在琼脂糖下对四种趋化因子（N-甲酰甲硫氨酰亮氨酰苯丙氨酸、补体片段C5a、白细胞介素-8和白三烯B4）的趋化反应、细胞表面Mac-1（CD11b/CD18）黏附受体的表达以及细胞质中肌动蛋白聚合的影响。从外周血中分离循环中的中性粒细胞，并从应用于健康受试者擦伤部位和烧伤患者皮肤移植供区的两种不同伤口敷料下收集的渗出液中分离渗出液中性粒细胞。烧伤损伤降低了循环中性粒细胞对所有四种趋化因子的趋化反应，提示趋化功能存在“整体”缺陷。在 Tegaderm 下收集的患者渗出液中性粒细胞的所有趋化反应进一步降低，而在 Biobrane 下收集的患者渗出液中性粒细胞则无运动能力。在 Biobrane 下收集的渗出液中性粒细胞表达高水平的Mac-1受体并不可逆地聚合肌动蛋白，这可能导致这些渗出液细胞的无运动能力。
