Braun W, Weber B, Moell U, Hamann A, Doerr H W
Department of Medical Virology, University Clinics of Frankfurt a. M., Germany.
J Virol Methods. 1993 Jun;43(1):65-75. doi: 10.1016/0166-0934(93)90090-e.
Immunoglobulin A and M patterns to human cytomegalovirus (HCMV) were investigated in sera from actively HCMV infected AIDS patients (n = 61) and healthy HCMV seropositive controls (n = 28) by a Western blot method (modi-blotting) and ELISA. The Western blot showed a higher detection rate for both IgA (78.7% vs. 52.5%) and IgM (83.6% vs. 13.1%) than ELISA in the immunocompromised patients suffering from active HCMV infection. Of the healthy seropositive individuals, 35.7% and 21.4% had a weak positive IgA- and IgM-class antibody reactivity respectively with HCMV-specific bands in the immunoblot. Immunoglobulins M and A were not detected by ELISA in the healthy control group. Immunoreactions in this group were restricted to viral polypeptides with M(r) of 68 kDa and 123 kDa. Additional bands were found in the actively infected patients only and were observed more frequently with IgA than IgM (47.5% vs. 29.5%). Results from the present study indicate, that using a sensitive Western blot technique, a higher serologic detection rate of active recurrent infection is achieved in AIDS patients. Nevertheless, immunoglobulin A and M are detected in a certain percentage of HCMV-seropositive healthy individuals not suffering from active HCMV infection. Broader immune reactions of HCMV-IgA as determined by HCMV Western blot assay are associated with an active infection, but were not present in all the actively infected patients.
采用蛋白质印迹法（改良印迹法）和酶联免疫吸附测定法（ELISA），对61例活动性人巨细胞病毒（HCMV）感染的艾滋病患者和28例HCMV血清学阳性的健康对照者血清中的HCMV免疫球蛋白A和M模式进行了研究。在活动性HCMV感染的免疫功能低下患者中，蛋白质印迹法检测到的IgA（78.7%对52.5%）和IgM（83.6%对13.1%）的检出率均高于ELISA。在健康血清学阳性个体中，分别有35.7%和21.4%的人在免疫印迹中与HCMV特异性条带的IgA和IgM类抗体反应呈弱阳性。健康对照组中ELISA未检测到免疫球蛋白M和A。该组的免疫反应仅限于分子量为68 kDa和123 kDa的病毒多肽。仅在活动性感染患者中发现了其他条带，且IgA出现的频率高于IgM（47.5%对29.5%）。本研究结果表明，使用灵敏的蛋白质印迹技术，艾滋病患者活动性复发感染的血清学检出率更高。然而，在一定比例的未患活动性HCMV感染的HCMV血清学阳性健康个体中检测到了免疫球蛋白A和M。HCMV蛋白质印迹分析确定的HCMV-IgA更广泛的免疫反应与活动性感染相关，但并非所有活动性感染患者都存在。
