Zhu Y, Ikeda S R
Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta 30912-2300.
Neurosci Lett. 1993 May 28;155(1):24-8. doi: 10.1016/0304-3940(93)90665-8.
Effects of the 'chemical phosphatase' 2,3-butanedione monoxide (BDM) on voltage-activated Ca2+ currents of adult rat superior cervical ganglion neurons were investigated using the whole-cell patch-clamp technique. BDM produced a rapid (< 10 s), reversible and dose-dependent (IC50 = 18.3 mM) inhibition of Ca2+ currents. The action of BDM was not prevented by 1 mM 8-(4-chlorophenylthio)-cAMP or 50 microM isoproterenol. H-7, a non-specific protein kinase inhibitor at 200 microM, did not prevent the rapid recovery from BDM-induced inhibition. Our results suggest that BDM inhibition of Ca2+ currents in rat sympathetic neurons does not require a 'chemical phosphatase' activity.
使用全细胞膜片钳技术研究了“化学磷酸酶”2,3-丁二酮一氧化物（BDM）对成年大鼠颈上神经节神经元电压激活钙电流的影响。BDM对钙电流产生快速（<10秒）、可逆且剂量依赖性（IC50 = 18.3 mM）的抑制作用。1 mM 8-（4-氯苯硫基）-环磷酸腺苷或50 μM异丙肾上腺素不能阻止BDM的作用。200 μM的非特异性蛋白激酶抑制剂H-7不能阻止BDM诱导的抑制作用的快速恢复。我们的结果表明，BDM对大鼠交感神经元钙电流的抑制作用不需要“化学磷酸酶”活性。
