Klebanoff S J, Kinsella M G, Wight T N
Department of Medicine, University of Washington, School of Medicine, Seattle.
Am J Pathol. 1993 Sep;143(3):907-17.
The degradation of the heparan sulfate proteoglycans of subendothelial matrix by neutrophil elastase and the myeloperoxidase-H2O2-chloride system added separately, sequentially, or together at pH 4.5 to 7.5 was determined by the release of lower molecular weight 35S-labeled material. Elastase alone and the myeloperoxidase system alone caused degradation, and when 4-hour exposure to elastase was followed by 15 minutes of exposure to the myeloperoxidase system, the effect was greater than additive. A greater than additive effect was not observed when elastase followed the myeloperoxidase system or the two were added together. Chloride (or sulfate) alone increased the release of 35S-labeled material from elastase-treated matrix, although the effect of 0.1 M chloride was not as great as that observed when an equivalent concentration of chloride was combined with myeloperoxidase and H2O2. The release of these systems at sites of adherence of neutrophils to glomerular basement membrane may contribute to neutrophil-associated proteinuria.
通过低分子量35S标记物质的释放，测定了在pH 4.5至7.5条件下，单独、依次或一起添加中性粒细胞弹性蛋白酶和髓过氧化物酶-H2O2-氯化物系统对内皮细胞下基质硫酸乙酰肝素蛋白聚糖的降解情况。单独的弹性蛋白酶和单独的髓过氧化物酶系统均可引起降解，当先让其接触弹性蛋白酶4小时，再接触髓过氧化物酶系统15分钟时，其效果大于两者相加的作用。当弹性蛋白酶在髓过氧化物酶系统之后加入或两者一起加入时，未观察到大于相加的作用。单独的氯化物（或硫酸盐）可增加弹性蛋白酶处理过的基质中35S标记物质的释放，尽管0.1M氯化物的作用不如同等浓度的氯化物与髓过氧化物酶和H2O2联合使用时观察到的作用大。这些系统在中性粒细胞黏附于肾小球基底膜部位的释放可能导致与中性粒细胞相关的蛋白尿。
