Kuwabara M, Nishimura Y, Sato F, Takahashi T, Tajima M, Takahashi K, Nagahata H
Department of Radiation Biology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Free Radic Res Commun. 1993;18(5):309-18. doi: 10.3109/10715769309147498.
The ability of neutrophils from a Holstein-Friesian calf with bovine leukocyte adhesion deficiency (the proband with a genetic deficiency of the Mac-1 (CD11b/CD18) glycoprotein corresponding to the receptor of complement iC3b) to generate oxygen radicals was examined using electron spin resonance spectrometry (ESR) combined with a spin-trapping technique and luminol-dependent chemiluminescence spectrometry. When the neutrophils were stimulated with phorbol 12-myristate 13-acetate (PMA), an ESR spectrum confirming the generation of superoxide anions (O2-) was clearly observed in both healthy and diseased calves. However, when the neutrophils were stimulated by opsonized zymosan, appearance of the ESR spectrum was recognized in the healthy calves but not in the diseased calf. Similar results were obtained from chemiluminescence experiments.
利用电子自旋共振光谱法（ESR）结合自旋捕获技术和鲁米诺依赖性化学发光光谱法，检测了一头患有牛白细胞粘附缺陷的荷斯坦 - 弗里生小牛（先证者，其Mac-1（CD11b/CD18）糖蛋白存在对应补体iC3b受体的基因缺陷）的中性粒细胞产生氧自由基的能力。当用佛波酯12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）刺激中性粒细胞时，在健康和患病小牛中均清晰观察到确认超氧阴离子（O2-）产生的ESR光谱。然而，当用调理酵母聚糖刺激中性粒细胞时，在健康小牛中可识别出ESR光谱的出现，而在患病小牛中则未观察到。化学发光实验也得到了类似结果。
