Sims C J, Fujito D T, Burholt D R, Dadok J, Giles H R, Wilkinson D A
Department of Obstetrics and Gynecology, Medical College of Pennsylvania/Allegheny Campus, Pittsburgh 15212-9986.
Prenat Diagn. 1993 Jun;13(6):473-80. doi: 10.1002/pd.1970130609.
We have investigated the ability of high-resolution proton NMR spectroscopy to provide a biochemical constituent screening of human amniotic fluid (AF). Proton NMR spectra were obtained at 300 MHz on AF from patients undergoing amniocentesis in the mid-trimester. Only AF from normal pregnancies (normal fetal karyotype, normal alpha-fetoprotein levels, normal birth outcome) was used in this study. The AF supernatant was lyophilized and resuspended in deuterated water containing 0.1 mM phosphate buffer and 6.02 mM disodium maleate. Identification of low molecular weight compounds was confirmed by two-dimensional NMR spectra (primarily correlated spectroscopy, or COSY) and standard addition techniques. A broad profile of compounds were 'NMR visible' in a single proton spectrum, including creatinine, glucose, organic acids (acetate, citrate, and lactate) and several amino acids (alanine, histidine, leucine, phenylalanine, tyrosine and valine). The proton spectrum was unaffected by prior freezing/thawing of AF samples. We were able to quantify compounds by comparison with an added concentration standard (maleate) at concentrations as low as 30 microM. Good agreement with literature values based on other analytical techniques was obtained.
我们研究了高分辨率质子核磁共振波谱法对人羊水（AF）进行生化成分筛查的能力。在妊娠中期对接受羊膜穿刺术患者的羊水，于300兆赫下获取质子核磁共振波谱。本研究仅使用来自正常妊娠（正常胎儿核型、正常甲胎蛋白水平、正常分娩结局）的羊水。羊水上清液经冻干后重悬于含有0.1毫摩尔磷酸盐缓冲液和6.02毫摩尔马来酸钠的重水中。通过二维核磁共振波谱（主要是相关波谱，即COSY）和标准加入技术确认低分子量化合物的鉴定。在单质子谱中可“通过核磁共振观察到”多种化合物，包括肌酐、葡萄糖、有机酸（乙酸盐、柠檬酸盐和乳酸盐）以及几种氨基酸（丙氨酸、组氨酸、亮氨酸、苯丙氨酸、酪氨酸和缬氨酸）。质子谱不受羊水样本先前冻融的影响。我们能够通过与添加的浓度标准物（马来酸盐）比较，对低至30微摩尔浓度的化合物进行定量。与基于其他分析技术的文献值取得了良好的一致性。
