研究笔记：一种检测罗氏相关内源性单个长末端重复序列ev15的快速方法。
Research note: a rapid method for the detection of the Rous-associated endogenous solitary long terminal repeat, ev15.
作者信息
Benkel B F, Smith E J
机构信息
Agriculture Canada, Centre for Food and Animal Research, Ottawa, Ontario.
出版信息
Poult Sci. 1993 Aug;72(8):1601-5. doi: 10.3382/ps.0721601.
A rapid method has been developed for the detection of the solitary long terminal repeat ev15, a member of the avian leukosis virus (ALV) family of endogenous viral elements (ev genes) in chickens. Detection is accomplished by a polymerase chain reaction (PCR) assay that can be performed on purified genomic DNA samples or crude preparations of partially purified whole blood lysates. The test discriminates unambiguously between birds that are homozygous ev15-, homozygous ev15+, or heterozygous ev15-/ev15+. The incorporation of a modified touchdown amplification profile significantly improved the specificity of the PCR assay. Small-scale screening of birds from a variety of chicken breeds has revealed that ev15 is present in populations of both egg-strain birds and broilers.
已开发出一种快速检测鸡内源性病毒元件（ev基因）禽白血病病毒（ALV）家族成员单独长末端重复序列ev15的方法。检测通过聚合酶链反应（PCR）测定法完成，该测定法可对纯化的基因组DNA样本或部分纯化的全血裂解物的粗制制剂进行。该测试能够明确区分纯合ev15-、纯合ev15+或杂合ev15-/ev15+的鸟类。采用改良的降落扩增曲线显著提高了PCR测定法的特异性。对来自各种鸡品种的鸟类进行小规模筛查发现，蛋鸡和肉鸡群体中均存在ev15。
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