长期摄入乙醇会降低佛波酯与大鼠脑组织膜蛋白激酶C的结合，但不会降低其与胞质蛋白激酶C的结合。

Chronic ethanol consumption decreases the phorbol ester binding to membranal but not cytosolic protein kinase C in rat brain.

作者信息

Pandey S C, Dwivedi Y, Piano M R, Schwertz D W, Davis J M, Pandey G N

机构信息

Illinois State Psychiatric Institute, University of Illinois, Chicago 60651.

出版信息

Alcohol. 1993 Jul-Aug;10(4):259-62. doi: 10.1016/0741-8329(93)90002-6.

DOI:10.1016/0741-8329(93)90002-6

PMID:8397875

Abstract

We examined the effect of 60 days of ethanol treatment on protein kinase C (PKC) in membrane and cytosolic fractions of the rat cerebral cortex. Membranal and cytosolic PKC were determined by binding technique using [3H]-phorbol 12,13 dibutyrate (PDBU) as radioligand and phorbol 12-myristate 13-acetate (PMA) as displacer. Chronic ethanol consumption resulted in a decrease in the maximum number of binding sites (Bmax) of [3H]-PDBU binding to membranal PKC without significant change in the apparent dissociation constant (KD) in the rat cortex. We also observed that chronic ethanol consumption had no significant effect on Bmax or KD of [3H]-PDBU binding to cytosolic PKC in the rat cerebral cortex. These results suggest that chronic ethanol consumption leads to the down-regulation of brain PKC associated with membrane but not with cytosol.

摘要

我们研究了60天乙醇处理对大鼠大脑皮质膜和胞质组分中蛋白激酶C（PKC）的影响。使用[3H]-佛波醇12,13-二丁酸酯（PDBU）作为放射性配体，佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）作为置换剂，通过结合技术测定膜和胞质中的PKC。长期摄入乙醇导致大鼠皮质膜PKC上[3H]-PDBU结合位点的最大数量（Bmax）减少，而表观解离常数（KD）无显著变化。我们还观察到，长期摄入乙醇对大鼠大脑皮质胞质PKC上[3H]-PDBU结合的Bmax或KD无显著影响。这些结果表明，长期摄入乙醇会导致与膜相关而非与胞质相关的脑PKC下调。