Gengyo-Ando K, Kamiya Y, Yamakawa A, Kodaira K, Nishiwaki K, Miwa J, Hori I, Hosono R
Department of Biochemistry, School of Medicine, Kanazawa University, Japan.
Neuron. 1993 Oct;11(4):703-11. doi: 10.1016/0896-6273(93)90080-b.
The C. elegans unc-18 gene is required to maintain normal acetylcholine levels. We determined the complete structure of an unc-18 cDNA that encodes a protein of 591 highly charged and hydrophilic amino acids. The protein shows sequence similarity with elements of the secretory pathway in the yeast S. cerevisiae. Antibodies raised against a portion of the unc-18-encoded protein (UNC-18) detected a 68 kd soluble antigen on immunoblots and intensely stained all vertical cord motor neurons in situ. These findings suggest that UNC-18 participates in the axonal transport system and influences the acetylcholine flow in motor neurons.
秀丽隐杆线虫的unc-18基因是维持正常乙酰胆碱水平所必需的。我们确定了一个unc-18 cDNA的完整结构，该cDNA编码一种由591个高度带电且亲水性氨基酸组成的蛋白质。该蛋白质与酿酒酵母分泌途径中的元件具有序列相似性。针对unc-18编码蛋白（UNC-18）的一部分产生的抗体在免疫印迹上检测到一种68 kd的可溶性抗原，并在原位强烈染色所有垂直索运动神经元。这些发现表明UNC-18参与轴突运输系统并影响运动神经元中的乙酰胆碱流动。
