Hsu S F, Burnette R R
School of Pharmacy, University of Wisconsin, Madison 53706.
Biochim Biophys Acta. 1993 Oct 10;1152(1):189-91. doi: 10.1016/0005-2736(93)90247-w.
By using the whole-cell patch technique, it is shown that the total outward current is increased, as a function of time, after the addition of amphotericin B to the bathing solution. The whole-cell current is shown to be primarily a K-channel current by the blockage of this current upon application of TEA to the bathing solution. Single K-channel studies, using the outside-out patch-clamp technique, reveal that the single K-channel opening probability increases by a factor of six after the addition of amphotericin B. In addition, single K-channel voltage dependent studies, using the inside-out patch-clamp technique, demonstrate that this increase in opening probability is due to an increase in the amplitude of Po(v). In contrast to the present belief that amphotericin B simply creates pores in a cell's membrane, these results suggest that amphotericin B can also influence the function of the cell's K-channel proteins.
通过使用全细胞膜片钳技术发现，在浴液中加入两性霉素B后，总的外向电流随时间增加。当向浴液中加入TEA阻断该电流时，全细胞电流显示主要为钾通道电流。使用外向膜片钳技术进行的单钾通道研究表明，加入两性霉素B后，单钾通道开放概率增加了6倍。此外，使用内向膜片钳技术进行的单钾通道电压依赖性研究表明，开放概率的增加是由于Po(v)幅度的增加。与目前认为两性霉素B只是在细胞膜上形成孔道的观点相反，这些结果表明两性霉素B也可以影响细胞钾通道蛋白的功能。
