Bai G, Kusiak J W
National Institute on Aging, National Institutes of Health, Molecular Neurobiology Unit, Baltimore, MD 21224.
Biochim Biophys Acta. 1993 Oct 10;1152(1):197-200. doi: 10.1016/0005-2736(93)90249-y.
We cloned and analyzed a 3.8 kb EcoRI fragment of the rat NMDAR1 gene. It contains 3 kb of promoter/enhancer region, exon 1 and a portion of intron 1. Two major transcription start sites were identified at -276 and -238 from the first nucleotide in codon 1. One GSG and two SP1 motifs, but no TATA/CAAT boxes, exist in the region proximal to the transcription start sites. Our results suggest that NMDAR1 has the characteristics of a housekeeping gene and may be regulated by immediate-early genes.
我们克隆并分析了大鼠NMDAR1基因的一个3.8 kb的EcoRI片段。它包含3 kb的启动子/增强子区域、外显子1和内含子1的一部分。在密码子1的第一个核苷酸上游-276和-238处鉴定出两个主要转录起始位点。在转录起始位点近端区域存在一个GSG基序和两个SP1基序，但没有TATA/CAAT框。我们的结果表明，NMDAR1具有管家基因的特征，可能受即早基因调控。
