蛋白聚糖对体外羟基磷灰石生长的影响：透明质酸的作用

Effect of proteoglycans on hydroxyapatite growth in vitro: the role of hyaluronan.

作者信息

Paschalakis P, Vynios D H, Tsiganos C P, Dalas E, Maniatis C, Koutsoukos P G

机构信息

Laboratory of Biochemistry, University of Patras, Greece.

出版信息

Biochim Biophys Acta. 1993 Oct 3;1158(2):129-36. doi: 10.1016/0304-4165(93)90006-t.

DOI:10.1016/0304-4165(93)90006-t

PMID:8399313

Abstract

The effect of cartilage proteoglycans on HA seed crystal growth was studied using a system providing constant supersaturation with respect to HA. The monomers were much less effective than the aggregates in reducing the rate of HA growth, which correlates with their affinity for the HA crystals. Hyaluronan, which is a normal constituent of the proteoglycan aggregates, behaved as a strong inhibitor of HA seed crystal growth and had an affinity constant similar to that of proteoglycan aggregates. The results indicate that inhibition of HA seed crystal growth is mediated through the interaction of hyaluronan with HA crystal surface and that the proteoglycans add to the volume of the adsorbate causing steric hindrance.

摘要

利用一个能提供相对于羟基磷灰石（HA）恒定过饱和度的系统，研究了软骨蛋白聚糖对HA籽晶生长的影响。单体在降低HA生长速率方面比聚集体的效果要差得多，这与其对HA晶体的亲和力相关。作为蛋白聚糖聚集体正常成分的透明质酸，表现为HA籽晶生长的强抑制剂，并且其亲和常数与蛋白聚糖聚集体的相似。结果表明，HA籽晶生长的抑制是通过透明质酸与HA晶体表面的相互作用介导的，并且蛋白聚糖增加了吸附物的体积从而造成空间位阻。