Wang S Y, Johnson P A
Department of Animal Science, Cornell University, Ithaca, New York 14853.
Biol Reprod. 1993 Sep;49(3):453-8. doi: 10.1095/biolreprod49.3.453.
Using a porcine inhibin alpha-subunit cDNA as a probe, we have isolated four inhibin alpha-subunit cDNA clones (cINA6, cINA7, cINA9, and cINA12) from a cDNA library constructed from chicken ovarian granulosa layer mRNA. These four clones shared a common 3' end, and cINA6 (1585 bp) contains a complete open reading frame encoding for chicken inhibin alpha-subunit. The chicken inhibin alpha-subunit is predicted to be synthesized as a large precursor of 328 amino acids including a signal peptide region and several possible proteolytic sites. The mature alpha-subunit is the carboxyl-terminal fragment of 113 amino acids containing one glycosylation site and seven cysteine residues. Northern analysis of total RNA from various tissues revealed that chicken inhibin alpha-subunit mRNA is approximately 1.7 kb. It is present in the ovarian granulosa layer but not in the brain, kidney, liver, or spleen. The cDNA and its predicted polypeptide of chicken inhibin alpha-precursor are considerably homologous to their mammalian counterparts (86-89% and 61-63%, respectively).
我们以猪抑制素α亚基cDNA为探针，从由鸡卵巢颗粒层mRNA构建的cDNA文库中分离出四个抑制素α亚基cDNA克隆（cINA6、cINA7、cINA9和cINA12）。这四个克隆具有共同的3'末端，cINA6（1585 bp）包含一个编码鸡抑制素α亚基的完整开放阅读框。鸡抑制素α亚基预计作为一个由328个氨基酸组成的大前体合成，包括一个信号肽区域和几个可能的蛋白水解位点。成熟的α亚基是由113个氨基酸组成的羧基末端片段，含有一个糖基化位点和七个半胱氨酸残基。对来自各种组织的总RNA进行的Northern分析表明，鸡抑制素α亚基mRNA约为1.7 kb。它存在于卵巢颗粒层中，但不存在于脑、肾、肝或脾中。鸡抑制素α前体的cDNA及其预测的多肽与它们的哺乳动物对应物具有相当高的同源性（分别为86 - 89%和61 - 63%）。
