Grachev S V, Eliseeva S V, Egorova N D, Nasedkin A N, Novikova A N, Prokhorov A M, Denker B I
Biull Eksp Biol Med. 1993 Jul;116(7):96-100.
A comparative evaluation has been carried out between the effects of surgical CO2-laser (lambda = 10.6 mcm) and lasers previously unused in medicine: Yag-erbium (lambda = 2.94 mcm) and YAG-erbium-in-glass lasers on biological tissue. The cutting properties of the lasers, nature of their effect on tissue and the period of healing of laser injury were studied morphologically. Effects of radiation on the energetic, biosynthetic and proliferative processes in the liver tissue, as well as the activity of transamination enzymes have been investigated. It has been shown that laser radiation of lambda = 1.54 and lambda = 2.94 mcm can destroy biological tissue with minimum inflammatory reaction as compared to CO2-laser. It has been ascertained that better marked changes in bioenergetics, aminotransferase activity, RNA and DNA contents are observed under the influence of CO2 and YAG-erbium lasers. Exposure of liver to YAG-erbium-in-glass laser radiation produced minimum changes in cells of the tissue contiguous to the injury. Thus, under its influence the majority of the investigated indices either changed insignificantly or improved. This might account for a positive effect of this laser on the dynamics and quality of the injury healing. The experimental studies gave basis for creation of working models of laser devices (lambda = 1.54 mcm) which are undergoing successful clinical trials at present.
已对外科用二氧化碳激光（波长λ = 10.6微米）与医学上以前未使用过的激光：掺铒钇铝石榴石激光（波长λ = 2.94微米）和玻璃中掺铒钇铝石榴石激光对生物组织的作用进行了比较评估。从形态学角度研究了这些激光的切割特性、它们对组织的作用性质以及激光损伤的愈合期。研究了辐射对肝组织中能量、生物合成和增殖过程的影响，以及转氨酶的活性。结果表明，与二氧化碳激光相比，波长λ = 1.54微米和λ = 2.94微米的激光辐射能够以最小的炎症反应破坏生物组织。已确定在二氧化碳激光和掺铒钇铝石榴石激光的影响下，生物能量学、氨基转移酶活性、RNA和DNA含量出现更明显的变化。肝组织暴露于玻璃中掺铒钇铝石榴石激光辐射下，损伤邻近组织的细胞产生的变化最小。因此，在其影响下，大多数研究指标要么变化不显著，要么有所改善。这可能解释了这种激光对损伤愈合的动态过程和质量具有积极作用。实验研究为创建激光设备（波长λ = 1.54微米）的工作模型提供了依据，目前该模型正在进行成功的临床试验。
