Macedo A M, Vallejo G A, Chiari E, Pena S D
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, Brazil.
EXS. 1993;67:321-9. doi: 10.1007/978-3-0348-8583-6_29.
Very little is known about the structure and sequence of the genomic DNA and kDNA of T. rangeli and no highly polymorphic markers are known. In this paper, we show that the Jeffreys' multilocal probe 33.15 produces characteristic DNA fingerprints with these trypanosomes. The multiband patterns can be used to differentiate T. cruzi from T. rangeli and for recognizing relationships between strains of the latter from widely different geographic areas and different hosts. The topology of a UPGMA phenetic tree constructed from band-sharing data suggests the existence of two groups of T. rangeli: one encompassing parasites from Central America and the northern part of South America and another with the parasites from southern Brazil. This splitting was confirmed by the use of both nuclear and kinetoplast unique sequence probes. Among strains of T. rangeli, band sharing was generally negatively correlated with geographical distance. This work confirms the usefulness of DNA fingerprints as a potent technique for the analysis of relationships in trypanosomatid populations.
关于兰氏锥虫基因组DNA和动基体DNA（kDNA）的结构和序列，人们了解甚少，也不存在已知的高度多态性标记。在本文中，我们展示了杰弗里斯氏多位点探针33.15能与这些锥虫产生特征性的DNA指纹图谱。多带型模式可用于区分克氏锥虫和兰氏锥虫，并识别来自广泛不同地理区域和不同宿主的兰氏锥虫菌株之间的关系。根据条带共享数据构建的UPGMA聚类树拓扑结构表明存在两组兰氏锥虫：一组包括来自中美洲和南美洲北部的寄生虫，另一组包括来自巴西南部的寄生虫。通过使用核基因和动基体独特序列探针证实了这种分化。在兰氏锥虫菌株中，条带共享通常与地理距离呈负相关。这项工作证实了DNA指纹图谱作为分析锥虫种群关系的有效技术的实用性。
