Fu C J, Zhao G S, Zhang J F
Dept. of Pathophysiology, Henan Medical University, Zhengzhou.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 1993 Apr;13(4):228-9, 198.
In recent years, it is believed by some scholars that the injury of myocardial ischemic reperfusion is correlated to the thromboxane A2 (TXA2) released by platelets. In order to explore that whether the myocardial and hemangio-endothelial cells participate in the TXA2 production during the process of reperfusion, the modified Langendorff method was used to establish the model of reperfusing the isolated rat heart. On the other hand, this experiment was also intended to observe the effect of ligustrazine on the injury of myocardial ischemic reperfusion. The results revealed that the level of thromboxane B2 (metabolite of TXA2) and lactic dehydrogenase (LDH) in coronary sinus reflux fluid increased during the process of reperfusion, while the level of 6-keto-PGF1 alpha in the same fluid relatively decreased (P < 0.05). The ratio of TXB2/6-keto-PGF1 alpha was raised. The ligustrazine inhibited the release of TXB2 and LDH, but promoted the production of 6-keto-PGF1 alpha (P < 0.05). The results also proved that the myocardial and hemangio-endothelial cells could synthesize TXA2, and the amount of TXA2 released increased during the reperfusion of ischemic myocardium, which was likely to be the major factor of the injury of ischemic myocardial reperfusion. Ligustrazine plays an important role in protecting the myocardium.
近年来，一些学者认为心肌缺血再灌注损伤与血小板释放的血栓素A2（TXA2）有关。为了探讨心肌和血管内皮细胞在再灌注过程中是否参与TXA2的产生，采用改良Langendorff法建立离体大鼠心脏再灌注模型。另一方面，本实验还旨在观察川芎嗪对心肌缺血再灌注损伤的影响。结果显示，再灌注过程中冠状窦回流液中血栓素B2（TXA2的代谢产物）和乳酸脱氢酶（LDH）水平升高，而同一液体中6-酮-前列腺素F1α水平相对降低（P<0.05）。TXB2/6-酮-前列腺素F1α比值升高。川芎嗪抑制TXB2和LDH的释放，但促进6-酮-前列腺素F1α的产生（P<0.05）。结果还证明，心肌和血管内皮细胞能够合成TXA2，缺血心肌再灌注时释放的TXA2量增加，这可能是缺血心肌再灌注损伤的主要因素。川芎嗪在保护心肌方面起重要作用。
