人胎盘中UDP-半乳糖：乳糖基神经酰胺α-半乳糖基转移酶活性
UDP-galactose:lactosylceramide alpha-galactosyltransferase activity in human placenta.
作者信息
Lampio A, Airaksinen A, Maaheimo H
机构信息
Department of Biochemistry, University of Helsinki, Finland.
出版信息
Glycoconj J. 1993 Apr;10(2):165-9. doi: 10.1007/BF00737713.
PMID:8400825
Abstract
The activity of UDP-Gal:LacCer galactosyltransferase in human placenta was studied by using crude homogenate and Triton CF-54 extract as the source of enzyme. Transfer of galactose to lactosylceramide was optimal in the presence of 0.1% Triton CF-54 and Mn2+ at pH 6.3, and the reaction product was susceptible to alpha-galactosidase.
摘要
利用粗匀浆和Triton CF - 54提取物作为酶源，对人胎盘组织中UDP - 半乳糖：乳糖神经酰胺半乳糖基转移酶的活性进行了研究。在pH 6.3条件下，0.1% Triton CF - 54和Mn2+存在时，半乳糖向乳糖神经酰胺的转移最为理想，且反应产物对α - 半乳糖苷酶敏感。
Zotero 插件