• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

A simple and sensitive experiment for measurement of JCC couplings between backbone carbonyl and methyl carbons in isotopically enriched proteins.

作者信息

Grzesiek S, Vuister G W, Bax A

机构信息

Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.

出版信息

J Biomol NMR. 1993 Jul;3(4):487-93. doi: 10.1007/BF00176014.

DOI:10.1007/BF00176014
PMID:8400833
Abstract

A simple 2D difference experiment is described that allows quantitative measurement of 13C-13C J couplings between backbone carbonyl and side-chain carbons. Precise 3JCC values were measured from data recorded in just 2 h for a 1-mM solution of the 20-kD complex between the protein calmodulin and a 26-residue synthetic peptide. The J couplings aid in determining the chi 1 angles of valine, isoleucine and threonine residues, and in making stereospecific assignments of the Val C gamma methyl groups. Error analysis indicates that the uncertainty in the derived J couplings is generally less than ca. 0.3 Hz.

摘要

相似文献

1
A simple and sensitive experiment for measurement of JCC couplings between backbone carbonyl and methyl carbons in isotopically enriched proteins.
J Biomol NMR. 1993 Jul;3(4):487-93. doi: 10.1007/BF00176014.
2
Chi 1 angle information from a simple two-dimensional NMR experiment that identifies trans 3JNC gamma couplings in isotopically enriched proteins.来自简单二维核磁共振实验的Chi 1角信息，该实验可识别同位素富集蛋白质中的反式3JNCγ偶合。
J Biomol NMR. 1997 Apr;9(3):323-8. doi: 10.1023/a:1018691228238.
3
Side chain and backbone assignments in isotopically labeled proteins from two heteronuclear triple resonance experiments.通过两个异核三重共振实验对同位素标记蛋白质中的侧链和主链进行归属。
FEBS Lett. 1992 Dec 21;314(3):413-8. doi: 10.1016/0014-5793(92)81517-p.
4
Measurement of two- and three-bond 13C-1H J couplings to the C delta carbons of leucine residues in staphylococcal nuclease.测量葡萄球菌核酸酶中亮氨酸残基的Cδ碳原子的二键和三键13C-1H J耦合。
J Biomol NMR. 1993 May;3(3):297-306. doi: 10.1007/BF00212516.
5
Measurement of two-bond JCOH alpha coupling constants in proteins uniformly enriched with 13C.在均匀富集13C的蛋白质中测量双键JCOHα耦合常数。
J Biomol NMR. 1992 Jul;2(4):401-5. doi: 10.1007/BF01874818.
6
Determination of methyl 13C-15N dipolar couplings in peptides and proteins by three-dimensional and four-dimensional magic-angle spinning solid-state NMR spectroscopy.通过三维和四维魔角旋转固态核磁共振光谱法测定肽和蛋白质中的甲基13C-15N偶极耦合。
J Chem Phys. 2008 Feb 7;128(5):052314. doi: 10.1063/1.2817638.
7
Measurement of three-bond, 13C'-13C beta J couplings in human ubiquitin by a triple resonance, E. COSY-type NMR technique.
J Biomol NMR. 1998 Feb;11(2):199-203. doi: 10.1023/a:1008241904271.
8
Measurement of HN-H alpha J couplings in calcium-free calmodulin using new 2D and 3D water-flip-back methods.使用新的二维和三维水反转回波方法测量无钙钙调蛋白中的HN-Hα J耦合。
J Biomol NMR. 1994 Nov;4(6):871-8. doi: 10.1007/BF00398416.
9
A three-dimensional NMR experiment with improved sensitivity for carbonyl-carbonyl J correlation in proteins.一种用于蛋白质中羰基-羰基J相关的灵敏度得到提高的三维核磁共振实验。
J Biomol NMR. 1997 Feb;9(2):207-11. doi: 10.1023/a:1018614505948.
10
Insights into the mobility of methyl-bearing side chains in proteins from (3)J(CC) and (3)J(CN) couplings.通过（3）J（CC）和（3）J（CN）耦合对蛋白质中含甲基侧链流动性的洞察。
J Am Chem Soc. 2003 Jul 23;125(29):8959-66. doi: 10.1021/ja029972s.

引用本文的文献

1
Structural basis for the transmembrane signaling and antidepressant-induced activation of the receptor tyrosine kinase TrkB.跨膜信号转导和受体酪氨酸激酶 TrkB 激活的结构基础：抗抑郁药的作用机制。
Nat Commun. 2024 Oct 29;15(1):9316. doi: 10.1038/s41467-024-53710-7.
2
Structural transitions enable interleukin-18 maturation and signaling.结构转变使白细胞介素-18 成熟和信号转导成为可能。
Immunity. 2024 Jul 9;57(7):1533-1548.e10. doi: 10.1016/j.immuni.2024.04.015. Epub 2024 May 10.
3
Structural basis for the ligand promiscuity of the neofunctionalized, carotenoid-binding fasciclin domain protein AstaP.

本文引用的文献

1
Interaction of calmodulin and a calmodulin-binding peptide from myosin light chain kinase: major spectral changes in both occur as the result of complex formation.钙调蛋白与肌球蛋白轻链激酶的一种钙调蛋白结合肽的相互作用：两者形成复合物时都会发生主要光谱变化。
Biochemistry. 1985 Dec 31;24(27):8152-7. doi: 10.1021/bi00348a047.
2
Solution structure of a calmodulin-target peptide complex by multidimensional NMR.通过多维核磁共振解析钙调蛋白-靶肽复合物的溶液结构
Science. 1992 May 1;256(5057):632-8. doi: 10.1126/science.1585175.
3
Target enzyme recognition by calmodulin: 2.4 A structure of a calmodulin-peptide complex.
新功能化的类胡萝卜素结合 fasciclin 域蛋白 AstaP 配体混杂的结构基础。
Commun Biol. 2023 Apr 28;6(1):471. doi: 10.1038/s42003-023-04832-z.
4
The architecture of transmembrane and cytoplasmic juxtamembrane regions of Toll-like receptors.Toll 样受体跨膜区和胞质近膜区的结构。
Nat Commun. 2023 Mar 17;14(1):1503. doi: 10.1038/s41467-023-37042-6.
5
NMR structure of emfourin, a novel protein metalloprotease inhibitor: Insights into the mechanism of action.恩福林的 NMR 结构，一种新型的蛋白金属蛋白酶抑制剂：作用机制的见解。
J Biol Chem. 2023 Apr;299(4):104585. doi: 10.1016/j.jbc.2023.104585. Epub 2023 Mar 6.
6
Spatial Structure of NanoFAST in the Apo State and in Complex with its Fluorogen HBR-DOM2.纳米 FAST 在 apo 态和与荧光团 HBR-DOM2 复合物中的空间结构。
Int J Mol Sci. 2022 Sep 26;23(19):11361. doi: 10.3390/ijms231911361.
7
Modulation of Toll-like receptor 1 intracellular domain structure and activity by Zn ions.锌离子对 Toll 样受体 1 胞内结构域的结构和活性的调节。
Commun Biol. 2021 Aug 24;4(1):1003. doi: 10.1038/s42003-021-02532-0.
8
Determining isoleucine side-chain rotamer-sampling in proteins from C chemical shift.从 C 化学位移确定蛋白质中异亮氨酸侧链构象的抽样。
Chem Commun (Camb). 2019 Dec 7;55(94):14107-14110. doi: 10.1039/c9cc06496f. Epub 2019 Oct 23.
9
Specificity of the Redox Complex between Cytochrome P450 24A1 and Adrenodoxin Relies on Carbon-25 Hydroxylation of Vitamin-D Substrate.细胞色素 P450 24A1 和肾上腺皮质酮还原酶之间氧化还原复合物的特异性依赖于维生素-D 底物的 C-25 羟化。
Drug Metab Dispos. 2019 Sep;47(9):974-982. doi: 10.1124/dmd.119.087759. Epub 2019 Jul 9.
10
CARD domain of rat RIP2 kinase: Refolding, solution structure, pH-dependent behavior and protein-protein interactions.大鼠 RIP2 激酶的 CARD 结构域：复性、溶液结构、pH 值依赖性及蛋白-蛋白相互作用
PLoS One. 2018 Oct 23;13(10):e0206244. doi: 10.1371/journal.pone.0206244. eCollection 2018.
钙调蛋白对靶酶的识别：钙调蛋白-肽复合物的2.4埃结构。
Science. 1992 Aug 28;257(5074):1251-5. doi: 10.1126/science.1519061.
4
Quantitative measurement of small through-hydrogen-bond and 'through-space' 1H-113Cd and 1H-199Hg J couplings in metal-substituted rubredoxin from Pyrococcus furiosus.嗜热栖热菌金属取代型铁氧化还原蛋白中通过氢键和“空间”的小分子1H-113Cd和1H-199Hg J耦合的定量测量。
J Biomol NMR. 1992 Sep;2(5):527-33. doi: 10.1007/BF02192814.