Nelson A G, Wolf E G, Bradshaw P O, Hearon C M, Li B
Department of Kinesiology, Louisiana State University, Baton Rouge 70803.
Undersea Hyperb Med. 1993 Sep;20(3):189-96.
To test whether repeated HBO exposures would increase activity of skeletal muscle metabolic enzymes, 27 rabbits (3 groups) were exposed 90 min/day, 5 days/wk to either 100% O2 at 243 kPa (HBO), 100% O2 at 101 kPa (HIO), or 21% O2 at 101 kPa (CON). Four animals per group were killed after 2 wk treatment, and the remaining five per group were killed after 8 wk of treatment. Soleus, plantaris, and tibialis anterior muscles were removed, and the activities of adenylate kinase, alpha-glycerophosphate dehydrogenase, and citrate synthase were measured. After 8 wk there was no difference in enzyme activity between groups for either plantaris or tibialis anterior. In the soleus after 8 wk there was no difference between groups in adenylate kinase activity, but alpha-glycerophosphate dehydrogenase activity was 56% greater (P < 0.05) in HBO than in HIO and 50% greater than in CON, and citrate synthase activity in HBO was 24% greater (P < 0.05) than that in HIO and 36% greater than that in CON. Inasmuch as the soleus is a postural muscle, these results suggest that long-term HBO treatments can increase enzyme activity in an actively contracting muscle.
为了测试重复进行高压氧（HBO）暴露是否会增加骨骼肌代谢酶的活性，将27只兔子（3组）每天暴露90分钟，每周5天，分别暴露于243 kPa的100%氧气（HBO）、101 kPa的100%氧气（HIO）或101 kPa的21%氧气（CON）环境中。每组4只动物在治疗2周后处死，其余每组5只在治疗8周后处死。取出比目鱼肌、跖肌和胫骨前肌，测量腺苷酸激酶、α-甘油磷酸脱氢酶和柠檬酸合酶的活性。8周后，跖肌或胫骨前肌组间酶活性无差异。8周后，比目鱼肌组间腺苷酸激酶活性无差异，但HBO组的α-甘油磷酸脱氢酶活性比HIO组高56%（P<0.05），比CON组高50%，HBO组的柠檬酸合酶活性比HIO组高24%（P<0.05），比CON组高36%。由于比目鱼肌是一种姿势肌，这些结果表明长期HBO治疗可增加主动收缩肌肉中的酶活性。
