溶血性贫血网织红细胞中的珠蛋白链合成。血红蛋白伯克一例。
Globin chain synthesis in hemolytic anemia reticulocytes. A case of hemoglobin Burke.
作者信息
Suzuki H, Wada C, Kamata K, Takahashi E, Sato N, Kunitomo T
机构信息
Department of Biophysical Chemistry, Kitasato University School of Medicine, Kanagawa, Japan.
出版信息
Biochem Mol Biol Int. 1993 Jul;30(3):425-31.
Peripheral red blood cells from an anemic patient were incubated with [14C]Leu and the labeled globin chains were analyzed by CM-cellulose column chromatography in 8M urea. The radioactivity almost equal to that of beta chain emerged between beta and alpha chains. The newly appeared materials were found to be derived from an abnormal hemoglobin (Hb), Hb Burke [beta 107(G9)Gly to Arg], by the sequence analyses of abnormal globin chain and beta globin mRNA from the patient.
将贫血患者的外周血红细胞与[14C]亮氨酸一起孵育，然后在8M尿素中通过CM-纤维素柱色谱法分析标记的珠蛋白链。在β链和α链之间出现了几乎与β链放射性相同的放射性物质。通过对患者异常珠蛋白链和β珠蛋白mRNA的序列分析发现，新出现的物质来源于一种异常血红蛋白（Hb），即Hb Burke[β107(G9)甘氨酸突变为精氨酸]。
Zotero 插件