Ferretti G, Taus M, Dousset N, Solera M L, Valdiguié P, Curatola G
Institute of Biochemistry, Faculty of Medicine, Ancona, Italy.
Biochem Mol Biol Int. 1993 Jul;30(4):713-9.
The modifications of the physico-chemical properties of the high density lipoprotein (HDL) before and after in vitro induced oxidation by copper ions have been studied using the fluorescence polarization (Pf) of the phosphatidylcholine derivative of 1,6-diphenyl-1,3,5-hexatriene (DPH-PC) and of the cationic derivative (TMA-DPH). We have observed that HDL oxidation is associated with a decrease of the molecular order at the lipoprotein surface as demonstrated by the increase in Pf with respect to untreated HDL. Moreover in oxidized HDL the polarity-sensitive probe laurdan has shown a decrease of the polarity in its microenvironment. It has been suggested that a decrease in HDL fluidity would inhibit cholesterol reverse transport from peripheral tissues in form of HDL core cholesteryl esters. Peroxidation of HDL, if occurring in vivo, could contribute to the progress of atherogenesis by decreasing cholesterol efflux from peripheral tissues.
利用1,6 - 二苯基 - 1,3,5 - 己三烯磷脂酰胆碱衍生物（DPH - PC）和阳离子衍生物（TMA - DPH）的荧光偏振（Pf），研究了高密度脂蛋白（HDL）在体外被铜离子诱导氧化前后的物理化学性质变化。我们观察到，HDL氧化与脂蛋白表面分子有序性降低有关，这通过与未处理的HDL相比Pf增加得以证明。此外，在氧化的HDL中，对极性敏感的探针劳丹（laurdan）显示其微环境中的极性降低。有人提出，HDL流动性降低会抑制胆固醇以HDL核心胆固醇酯的形式从外周组织逆向转运。如果HDL在体内发生过氧化，可能会通过减少外周组织的胆固醇流出而促进动脉粥样硬化的发展。
