Ranadive G N, Rosenzweig H S, Epperly M W, Seskey T, Bloomer W D
Department of Radiation Oncology, University of Pittsburgh, PA 15213.
Nucl Med Biol. 1993 Aug;20(6):719-26. doi: 10.1016/0969-8051(93)90158-q.
We have developed a very efficient labeling technique for monoclonal antibodies with technetium-99m. Oxidation of sugar residues on the IgG class of antibodies leads to the generation of aldehyde groups which are further reacted with two newly developed hydrazide compounds. This methodology introduces sulfhydryl groups on the antibody through sugar residues which can be labeled with technetium-99m. We have studied the TAG-72 specific second generation antibody CC-49. The specific activity of the labeled antibody was high without loss of its immunoreactivity.
我们已经开发出一种用99m锝标记单克隆抗体的高效标记技术。抗体IgG类上糖残基的氧化会导致醛基的产生，醛基会与两种新开发的酰肼化合物进一步反应。这种方法通过糖残基在抗体上引入巯基，巯基可以用99m锝标记。我们研究了TAG-72特异性第二代抗体CC-49。标记抗体的比活性很高，且其免疫反应性没有丧失。
