Yoshimura T, Kurita C, Hayata M, Nagai H
Department of Pharmacy, Ogaki Municipal Hospital, Gifu, Japan.
Biol Pharm Bull. 1993 Jul;16(7):686-9. doi: 10.1248/bpb.16.686.
The lymphocyte stimulation test (LST) is one of the most useful laboratory tests for the identification of allergy to a specific drug. The present study was conducted to examine utility of the LST using the [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) assay as a method for diagnosing drug allergy. The basic experimental conditions for the MTT assay were determined by using CTLL cells from human T-lymphoma, dependent on IL-2, and human peripheral blood lymphocytes. The amount of MTT needed was 0.5 mg per well and the peak maximum of MTT formazan was close to 565 nm. The blastogenesis of the lymphocytes was expressed as a Stimulation Index (SI). The value of the SI was almost constant at cell numbers ranging from 5 x 10(5) to 1 x 10(6) cells/ml. The SI of some drugs using normal peripheral blood lymphocytes was less than 1.3. We used the MTT assay in the LST to examine the allergenicity of 83 drugs which were used in 43 cases of suspected drug-induced hepatitis. The range of SI was 0.92-2.02. An SI of 1.2 or more was seen in 62.8% of cases, 1.3 or more in 51.2% of cases, and 1.4 or more in 41.9% of cases. There were 26 drugs with SI greater than 1.3. Seven of these were antibiotics, while the rest included antihypertensives, analgesics, psychotropics, antiallergics and antiepileptics. This method is extremely well suited for the diagnosis of drug allergy in a clinical situation. The greatest benefit of the MTT assay is that the complete test procedures can be carried out in a general laboratory.
淋巴细胞刺激试验（LST）是鉴定对特定药物过敏最有用的实验室检测方法之一。本研究旨在通过使用[3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基溴化四氮唑]（MTT）法作为诊断药物过敏的方法来检验LST的效用。MTT法的基本实验条件是通过使用来自人T淋巴瘤、依赖白细胞介素 - 2的CTLL细胞和人外周血淋巴细胞来确定的。所需MTT的量为每孔0.5毫克，MTT甲臜的最大峰值接近565纳米。淋巴细胞的增殖以刺激指数（SI）表示。在细胞数量为5×10⁵至1×10⁶个细胞/毫升的范围内，SI值几乎恒定。使用正常外周血淋巴细胞时，某些药物的SI小于1.3。我们在LST中使用MTT法检测了43例疑似药物性肝炎患者使用的83种药物的致敏性。SI范围为0.92 - 2.02。62.8%的病例SI为1.2或更高，51.2%的病例SI为1.3或更高，41.9%的病例SI为1.4或更高。有26种药物的SI大于1.3。其中7种是抗生素，其余包括抗高血压药、镇痛药、精神药物、抗过敏药和抗癫痫药。这种方法非常适合临床情况下的药物过敏诊断。MTT法的最大优点是整个检测程序可以在普通实验室进行。
