咖啡因导致大鼠心室肌细胞内向整流钾电流和内向钙电流降低。
Caffeine-induced decreases in the inward rectifier potassium and the inward calcium currents in rat ventricular myocytes.
作者信息
Varro A, Hester S, Papp J G
机构信息
Department of Pharmacology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
出版信息
Br J Pharmacol. 1993 Aug;109(4):895-7. doi: 10.1111/j.1476-5381.1993.tb13702.x.
Abstract
The effects of high (20 mM) concentrations of caffeine were studied on the transmembrane voltage and currents in rat single ventricular myocytes by the whole cell configuration of the patch clamp technique. Rapid application of caffeine released Ca2+ from the sarcoplasmic reticulum and induced a Ni(2+)-sensitive transient inward current with concomitant change of the transmembrane voltage from -72.6 +/- 0.4 to -68.0 +/- 0.6 mV (n = 4). Maintained application of caffeine lengthened the action potential duration (APD90) from 66.7 +/- 16.9 to 135.1 +/- 34.1 ms (n = 4) and depressed the amplitude of both the inward rectifier potassium and the inward calcium currents. It is concluded that these effects of caffeine should be recognized when it is used as a tool to study electromechanical coupling.
摘要
采用膜片钳技术的全细胞记录模式，研究了高浓度（20 mM）咖啡因对大鼠单个心室肌细胞膜电压和电流的影响。快速施加咖啡因可使肌浆网释放Ca2+，并诱发镍（2+）敏感的瞬时内向电流，同时跨膜电压从-72.6±0.4 mV变为-68.0±0.6 mV（n = 4）。持续施加咖啡因可使动作电位时程（APD90）从66.7±16.9 ms延长至135.1±34.1 ms（n = 4），并降低内向整流钾电流和内向钙电流的幅度。得出结论，当咖啡因用作研究电机械偶联的工具时，应认识到其这些作用。
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